TY - JOUR ID - 1903 TI - Predictive value of hypoglycin A and methylencyclopropylacetic acid conjugates in a horse with atypical myopathy in comparison to its cograzing partners JO - Equine Vet Educ PY - 2018 SP - 24-28 AU - Bochnia, M. AU - Scheidemann, W. AU - Ziegler, J. AU - Sander, J. AU - Vollstedt, S. AU - Glatter, M. AU - Janzen, N. AU - Terhardt, M. AU - Zeyner, A. VL - 30 UR - http://onlinelibrary.wiley.com/journal/10.1001/(ISSN)2042-3292 DO - 10.1111/eve.12596 AB - Hypoglycin A (HGA) was detected in blood and urine of a horse suffering from atypical myopathy (AM; Day 2, serum, 8290 μg/l; urine: Day 1, 574, Day 2, 742 μg/l) and in its cograzing partners with a high variability (46–1570 μg/l serum). Over the period of disease, the level of the toxic metabolites (methylencyclopropylacetic acid [MCPA]-conjugates) increased in body fluids of the AM horse (MCPA-carnitine: Day 2, 0.246, Day 3, 0.581 μmol/l serum; MCPA-carnitine: Day 2, 0.621, Day 3, 0.884 μmol/mmol creatinine in urine) and HGA decreased rapidly (Day 3, 2430 μg/l serum). In cograzing horses MCPA-conjugates were not detected. HGA in seeds ranged from 268 to 367 μg/g. Although HGA was present in body fluids of healthy cograzing horses, MCPA-conjugates were not detectable, in contrast to the AM horse. Therefore, increasing concentrations of MCPA-conjugates are supposed to be linked with the onset of AM and both parameters seem to indicate the clinical stage of disease. However, detection of HGA in body fluids of cograzing horses might be a promising step in preventing the disease. A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 1304 TI - Phosphate sensing in root development JO - Curr Opin Plant Biol PY - 2011 SP - 303-309 AU - Abel, S. VL - 14 UR - https://dx.doi.org/10.1016/j.pbi.2011.04.007 DO - 10.1016/j.pbi.2011.04.007 AB - Phosphate (Pi) and its anhydrides constitute major nodes in metabolism. Thus, plant performance depends directly on Pi nutrition. Inadequate Pi availability in the rhizosphere is a common challenge to plants, which activate metabolic and developmental responses to maximize Pi usage and acquisition. The sensory mechanisms that monitor environmental Pi and transmit the nutritional signal to adjust root development have increasingly come into focus. Recent transcriptomic analyses and genetic approaches have highlighted complex antagonistic interactions between external Pi and Fe bioavailability and have implicated the stem cell niche as a target of Pi sensing to regulate root meristem activity. A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 854 TI - Auxin signaling JO - Curr Opin Plant Biol PY - 2006 SP - 448-453 AU - Quint, M. AU - Gray, W.M. VL - 9 UR - DO - 10.1016/j.pbi.2006.07.006 AB - Auxin regulates a host of plant developmental and physiological processes, including embryogenesis, vascular differentiation, organogenesis, tropic growth, and root and shoot architecture. Genetic and biochemical studies carried out over the past decade have revealed that much of this regulation involves the SCFTIR1/AFB-mediated proteolysis of the Aux/IAA family of transcriptional regulators. With the recent finding that the TRANSPORT INHIBITOR RESPONSE1 (TIR1)/AUXIN SIGNALING F-BOX (AFB) proteins also function as auxin receptors, a potentially complete, and surprisingly simple, signaling pathway from perception to transcriptional response is now before us. However, understanding how this seemingly simple pathway controls the myriad of specific auxin responses remains a daunting challenge, and compelling evidence exists for SCFTIR1/AFB-independent auxin signaling pathways. A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 701 TI - The wound response in tomato - Role of jasmonic acid JO - J. Plant Physiol PY - 2006 SP - 297-306 AU - Wasternack, C. AU - Stenzel, I. AU - Hause, B. AU - Hause, G. AU - Kutter, C. AU - Maucher, H. AU - Neumerkel, J. AU - Feussner, I. AU - Miersch, O. VL - 163 UR - DO - 10.1016/j.jplph.2005.10.014 AB - A2 - C1 - Molecular Signal Processing; Cell and Metabolic Biology ER - TY - JOUR ID - 1140 TI - Genome-wide comparative analysis of the IQD gene families in Arabidopsis thaliana and Oryza sativa JO - BMC Evolutionary Biology PY - 2005 SP - 72 (1-25) AU - Abel, S. AU - Savchenko, T. AU - Levy, M. VL - 5 UR - http://www.biomedcentral.com/1471-2148/5/72 AB - We identified and analyzed 33 and 29 IQD1-like genes in Arabidopsis thaliana and Oryza sativa, respectively. The encoded IQD proteins contain a plant-specific domain of 67 conserved amino acid residues, referred to as the IQ67 domain, which is characterized by a unique and repetitive arrangement of three different calmodulin recruitment motifs, known as the IQ, 1-5-10, and 1-8-14 motifs. We demonstrated calmodulin binding for IQD20, the smallest IQD protein in Arabidopsis, which consists of a C-terminal IQ67 domain and a short N-terminal extension. A striking feature of IQD proteins is the high isoelectric point (~10.3) and frequency of serine residues (~11%). We compared the Arabidopsis and rice IQD gene families in terms of gene structure, chromosome location, predicted protein properties and motifs, phylogenetic relationships, and evolutionary history. The existence of an IQD-like gene in bryophytes suggests that IQD proteins are an ancient family of calmodulin-binding proteins and arose during the early evolution of land plants. Comparative phylogenetic analyses indicate that the major IQD gene lineages originated before the monocot-eudicot divergence. The extant IQD loci in Arabidopsis primarily resulted from segmental duplication and reflect preferential retention of paralogous genes, which is characteristic for proteins with regulatory functions. Interaction of IQD1 and IQD20 with calmodulin and the presence of predicted calmodulin binding sites in all IQD family members suggest that IQD proteins are a new class of calmodulin targets. The basic isoelectric point of IQD proteins and their frequently predicted nuclear localization suggest that IQD proteins link calcium signaling pathways to the regulation of gene expression. Our comparative genomics analysis of IQD genes and encoded proteins in two model plant species provides the first step towards the functional dissection of this emerging family of putative calmodulin targets. A2 - C1 - Molecular Signal Processing ER -