TY - JOUR ID - 2420 TI - Auxin-jasmonate crosstalk in Oryza sativa L. root system formation after cadmium and/or arsenic exposure JO - Environ Exp Bot PY - 2019 SP - 59-69 AU - Ronzan, M. AU - Piacentini, D. AU - Fattorini, L. AU - Federica, D. R. AU - Caboni, E. AU - Eiche, E. AU - Ziegler, J. AU - Hause, B. AU - Riemann, M. AU - Betti, C. AU - Altamura, M. M. AU - Falasca, G. VL - 165 UR - https://dx.doi.org/10.1016/j.envexpbot.2019.05.013 DO - 10.1016/j.envexpbot.2019.05.013 AB - Soil pollutants may affect root growth through interactions among phytohormones like auxin and jasmonates. Rice is frequently grown in paddy fields contaminated by cadmium and arsenic, but the effects of these pollutants on jasmonates/auxin crosstalk during adventitious and lateral roots formation are widely unknown. Therefore, seedlings of Oryza sativa cv. Nihonmasari and of the jasmonate-biosynthetic mutant coleoptile photomorphogenesis2 were exposed to cadmium and/or arsenic, and/or jasmonic acid methyl ester, and then analysed through morphological, histochemical, biochemical and molecular approaches.In both genotypes, arsenic and cadmium accumulated in roots more than shoots. In the roots, arsenic levels were more than twice higher than cadmium levels, either when arsenic was applied alone, or combined with cadmium. Pollutants reduced lateral root density in the wild -type in every treatment condition, but jasmonic acid methyl ester increased it when combined with each pollutant. Interestingly, exposure to cadmium and/or arsenic did not change lateral root density in the mutant. The transcript levels of OsASA2 and OsYUCCA2, auxin biosynthetic genes, increased in the wild-type and mutant roots when pollutants and jasmonic acid methyl ester were applied alone. Auxin (indole-3-acetic acid) levels transiently increased in the roots with cadmium and/or arsenic in the wild-type more than in the mutant. Arsenic and cadmium, when applied alone, induced fluctuations in bioactive jasmonate contents in wild-type roots, but not in the mutant. Auxin distribution was evaluated in roots of OsDR5::GUS seedlings exposed or not to jasmonic acid methyl ester added or not with cadmium and/or arsenic. The DR5::GUS signal in lateral roots was reduced by arsenic, cadmium, and jasmonic acid methyl ester. Lipid peroxidation, evaluated as malondialdehyde levels, was higher in the mutant than in the wild-type, and increased particularly in As presence, in both genotypes.Altogether, the results show that an auxin/jasmonate interaction affects rice root system development in the presence of cadmium and/or arsenic, even if exogenous jasmonic acid methyl ester only slightly mitigates pollutants toxicity. A2 - C1 - Cell and Metabolic Biology; Molecular Signal Processing ER - TY - JOUR ID - 1277 TI - Auxin Perception—Structural Insights JO - Cold Spring Harb Perspect Biol PY - 2010 SP - a005546 AU - Calderon-Villalobos, L. I. AU - Tan, X. AU - Zheng, N. AU - Estelle, M. VL - 2 UR - http://cshperspectives.cshlp.org/content/2/7/a005546.full?sid=084e458a-ebe2-449e-a254-577d24ee0e29 DO - 10.1101/cshperspect.a005546 AB - The identity of the auxin receptor(s) and the mechanism of auxin perception has been a subject of intense interest since the discovery of auxin almost a century ago. The development of genetic approaches to the study of plant hormone signaling led to the discovery that auxin acts by promoting degradation of transcriptional repressors called Aux/IAA proteins. This process requires a ubiquitin protein ligase (E3) called SCFTIR1 and related SCF complexes. Surprisingly, auxin works by directly binding to TIR1, the F-box protein subunit of this SCF. Structural studies demonstrate that auxin acts like a molecular glue, to stabilize the interaction between TIR1 and the Aux/IAA substrate. These exciting results solve an old problem in plant biology and reveal new mechanisms for E3 regulation and hormone perception. A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 1278 TI - Odyssey of Auxin JO - Cold Spring Harb Perspect Biol PY - 2010 SP - a004572 AU - Abel, S. AU - Theologis, A. VL - 2 UR - http://cshperspectives.cshlp.org/content/2/10/a004572.full.pdf+html?sid=2dcc6405-a286-4a24-a297-b935835ee65a DO - 10.1101/cshperspect.a004572 AB - The history of plant biology is inexorably intertwined with the conception and discovery of auxin, followed by the many decades of research to comprehend its action during growth and development. Growth responses to auxin are complex and require the coordination of auxin production, transport, and perception. In this overview of past auxin research, we limit our discourse to the mechanism of auxin action. We attempt to trace the almost epic voyage from the birth of the hormonal concept in plants to the recent crystallographic studies that resolved the TIR1-auxin receptor complex, the first structural model of a plant hormone receptor. The century-long endeavor is a beautiful illustration of the power of scientific reasoning and human intuition, but it also brings to light the fact that decisive progress is made when new technologies emerge and disciplines unite. A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 699 TI - Genetic transformation of barley to modify expression of a 13-lipoxygenase JO - Acta Biol. Szeged PY - 2005 SP - 33-34 AU - Sharma, V.K. AU - Monostori, T. AU - Hause, B. AU - Maucher, H. AU - Göbel, C. AU - Hornung, E. AU - Hänsch, R. AU - Bittner, F. AU - Wasternack, C. AU - Feussner, I. AU - Mendel, R.R. AU - Schulze, J. VL - 49 UR - http://www2.sci.u-szeged.hu/ABS/2005/Acta%20HP/4933.pdf AB - Immature scutella of barley were transformed with cDNA coding for a 13-li-poxygenase of barley (LOX-100) via particle bombardment. Regenerated plants were tested by PAT-assay, Western-analysis and PCR-screening. Immunocytochemical assay of T0 plants showed expression of the LOX cDNA both in the chloroplasts and in the cytosol, depending on the presence of the chloroplast signal peptide sequences in the cDNA. A few transgenic plants containing higher amounts of LOX-derived products have been found. These are the candidates for further analysis concerning pathogen resistance. A2 - C1 - Molecular Signal Processing; Cell and Metabolic Biology ER - TY - CHAP ID - 426 TI - Transcriptional activation of jasmonate biosynthesis enzymes is not reflected at protein level T2 - Advanced Research on Plant Lipids PB - Kluwer Academic Publishers PY - 2003 SP - 267-270 AU - Stenzel, I. AU - Hause, B. AU - Feussner, I. AU - Wasternack, C. VL - 0 UR - AB - A2 - Murata, N., Yamada, M., Nishida, I., Okuyama, H., Sekijar, J., Hajme, W. C1 - Molecular Signal Processing; Cell and Metabolic Biology ER - TY - CHAP ID - 428 TI - Shift in fatty acid and oxylipin pattern of tomato leaves following overexpression of the allene oxide cyclase T2 - Advanced Research on Plant Lipids PB - Kluwer Academic Publishers PY - 2003 SP - 275-278 AU - Weichert, H. AU - Maucher, H. AU - Hornung, E. AU - Wasternack, C. AU - Feussner, I. VL - 0 UR - AB - A2 - Murata, N., Yamada, M., Nishida, I., Okuyama, H., Sekijar, J., Hajme, W. C1 - Molecular Signal Processing ER -