@Article{IPB-2420, author = {Ronzan, M. and Piacentini, D. and Fattorini, L. and Federica, D. R. and Caboni, E. and Eiche, E. and Ziegler, J. and Hause, B. and Riemann, M. and Betti, C. and Altamura, M. M. and Falasca, G.}, title = {{Auxin-jasmonate crosstalk in Oryza sativa L. root system formation after cadmium and/or arsenic exposure}}, year = {2019}, pages = {59-69}, journal = {Environ Exp Bot}, doi = {10.1016/j.envexpbot.2019.05.013}, url = {https://dx.doi.org/10.1016/j.envexpbot.2019.05.013}, volume = {165}, abstract = {Soil pollutants may affect root growth through interactions among phytohormones like auxin and jasmonates. Rice is frequently grown in paddy fields contaminated by cadmium and arsenic, but the effects of these pollutants on jasmonates/auxin crosstalk during adventitious and lateral roots formation are widely unknown. Therefore, seedlings of Oryza sativa cv. Nihonmasari and of the jasmonate-biosynthetic mutant coleoptile photomorphogenesis2 were exposed to cadmium and/or arsenic, and/or jasmonic acid methyl ester, and then analysed through morphological, histochemical, biochemical and molecular approaches.In both genotypes, arsenic and cadmium accumulated in roots more than shoots. In the roots, arsenic levels were more than twice higher than cadmium levels, either when arsenic was applied alone, or combined with cadmium. Pollutants reduced lateral root density in the wild -type in every treatment condition, but jasmonic acid methyl ester increased it when combined with each pollutant. Interestingly, exposure to cadmium and/or arsenic did not change lateral root density in the mutant. The transcript levels of OsASA2 and OsYUCCA2, auxin biosynthetic genes, increased in the wild-type and mutant roots when pollutants and jasmonic acid methyl ester were applied alone. Auxin (indole-3-acetic acid) levels transiently increased in the roots with cadmium and/or arsenic in the wild-type more than in the mutant. Arsenic and cadmium, when applied alone, induced fluctuations in bioactive jasmonate contents in wild-type roots, but not in the mutant. Auxin distribution was evaluated in roots of OsDR5::GUS seedlings exposed or not to jasmonic acid methyl ester added or not with cadmium and/or arsenic. The DR5::GUS signal in lateral roots was reduced by arsenic, cadmium, and jasmonic acid methyl ester. Lipid peroxidation, evaluated as malondialdehyde levels, was higher in the mutant than in the wild-type, and increased particularly in As presence, in both genotypes.Altogether, the results show that an auxin/jasmonate interaction affects rice root system development in the presence of cadmium and/or arsenic, even if exogenous jasmonic acid methyl ester only slightly mitigates pollutants toxicity.} } @INBOOK{IPB-2417, author = {Möller, B. and Bürstenbinder, K.}, title = {{2019 IEEE 16th International Symposium on Biomedical Imaging (ISBI 2019)}}, year = {2019}, pages = {199-203}, chapter = {{Semi-Automatic Cell Segmentation from Noisy Image Data for Quantification of Microtubule Organization on Single Cell Level}}, doi = {10.1109/ISBI.2019.8759145}, url = {https://dx.doi.org/10.1109/ISBI.2019.8759145}, abstract = {The structure of the microtubule cytoskeleton provides valuable information related to morphogenesis of cells. The cytoskeleton organizes into diverse patterns that vary in cells of different types and tissues, but also within a single tissue. To assess differences in cytoskeleton organization methods are needed that quantify cytoskeleton patterns within a complete cell and which are suitable for large data sets. A major bottleneck in most approaches, however, is a lack of techniques for automatic extraction of cell contours. Here, we present a semi-automatic pipeline for cell segmentation and quantification of microtubule organization. Automatic methods are applied to extract major parts of the contours and a handy image editor is provided to manually add missing information efficiently. Experimental results prove that our approach yields high-quality contour data with minimal user intervention and serves a suitable basis for subsequent quantitative studies.} } @INBOOK{IPB-2102, author = {Flores, R. and Gago-Zachert, S. and De la Peña, M. and Navarro, B.}, title = {{Viroids and Satellite. - Academic Press}}, year = {2017}, pages = {331-338}, chapter = {{Chrysanthemum Chlorotic Mottle Viroid}}, editor = {Ed. A. Hadidi, et al.}, doi = {10.1016/B978-0-12-801498-1.00031-0}, url = {https://www.sciencedirect.com/science/article/pii/B9780128014981000310}, } @INBOOK{IPB-1928, author = {Wasternack, C.}, title = {{eLS}}, year = {2016}, chapter = {{Jasmonates: Synthesis, Metabolism, Signal Transduction and Action}}, doi = {10.1002/9780470015902.a0020138.pub2}, url = {https://dx.doi.org/10.1002/9780470015902.a0020138.pub2}, abstract = {Jasmonic acid and other fatty-acid-derived compounds called oxylipins are signals in stress responses and development of plants. The receptor complex, signal transduction components as well as repressors and activators in jasmonate-induced gene expression have been elucidated. Different regulatory levels and cross-talk with other hormones are responsible for the multiplicity of plant responses to environmental and developmental cues.} } @INBOOK{IPB-2566, author = {Parniske, M. and Ried, M.}, title = {{Die Sprache der Moleküle – Chemische Kommunikation in der Natur}}, year = {2016}, pages = {105-116}, chapter = {{Wahrnehmung und Interpretation symbiontischer Signale durch Pflanzen und ihre bakteriellen Partner}}, editor = {Deigele, C., ed.}, abstract = {Mutualistic symbioses between plant roots and microorganisms can reduce the demand for chemical fertilizers in agriculture. Most crops are able to establish arbuscular mycorrhiza (AM) symbiosis with fungi to take up phosphate more efficiently. A second symbiosis, nitrogen-fixing root nodule symbiosis, supersedes energy-intensive nitrogen fertilization: Legumes such as peas, clover and soybeans take up rhizobia – special bacteria that are capable of converting atmospheric nitrogen into ammonium – into their root cells. Plant root cells perceive rhizobia and AM fungi via very similar signaling molecules (N-acetylglucosamine tetra- or pentamers), even though the resultant developmental processes differ strongly. Interestingly, N-acetylglucosamine containing signals including fungal chitin- and bacterial peptidoglycan-fragments from their cell walls, also play a role in the recognition of pathogenic microorganisms.Despite the intrinsic sustainability potential of the nitrogen-fixing root nodule symbiosis, too much of a good thing, however, has led to global problems: The massive increase in global meat production is largely based on soybean. Large scale soybean monoculture destroyed ecosystems in South America. Large scale animal production results in excessive methane and nitrogen release into the environment, which causes climate change and death zones in marine ecosystems, respectively. This calls for a considerable reduction in meat consumption.} } @INBOOK{IPB-1718, author = {Tissier, A. and Ziegler, J. and Vogt, T.}, title = {{Ecological Biochemistry: Environmental and Interspecies Interactions}}, year = {2015}, pages = {14-37}, chapter = {{Specialized Plant Metabolites: Diversity and Biosynthesis}}, editor = {Krauss, G.-J. \& Nies, D. H., eds.}, doi = {10.1002/9783527686063.ch2}, url = {http://onlinelibrary.wiley.com/doi/10.1002/9783527686063.ch2/summary}, abstract = {Plant secondary metabolites, also termed specialized plant metabolites, currently comprise more than 200 000 natural products that are all based on a few biosynthetic pathways and key primary metabolites. Some pathways like flavonoid and terpenoid biosynthesis are universally distributed in the plant kingdom, whereas others like alkaloid or cyanogenic glycoside biosynthesis are restricted to a limited set of taxa. Diversification is achieved by an array of mechanisms at the genetic and enzymatic level including gene duplications, substrate promiscuity of enzymes, cell‐specific regulatory systems, together with modularity and combinatorial aspects. Specialized metabolites reflect adaptations to a specific environment. The observed diversity illustrates the heterogeneity and multitude of ecological habitats and niches that plants have colonized so far and constitutes a reservoir of potential new metabolites that may provide adaptive advantage in the face of environmental changes. The code that connects the observed chemical diversity to this ecological diversity is largely unknown. One way to apprehend this diversity is to realize its tremendous plasticity and evolutionary potential. This chapter presents an overview of the most widespread and popular secondary metabolites, which provide a definite advantage to adapt to or to colonize a particular environment, making the boundary between the “primary” and the “secondary” old fashioned and blurry.} } @INBOOK{IPB-1618, author = {Wasternack, C.}, title = {{Phytohormones: a window to metabolism, signaling and biotechnological applications.}}, year = {2014}, pages = {221-264}, chapter = {{Jasmonates in plant growth and stress responses.}}, editor = {Tran, L.-S.; Pal, S.}, doi = {10.1007/978-1-4939-0491-4_8}, url = {http://www.springer.com/de/book/9781493904907}, volume = {Springer}, abstract = {Abiotic and biotic stresses adversely affect plant growth and productivity. The phytohormones regulate key physiological events under normal and stressful conditions for plant development. Accumulative research efforts have discovered important roles of phytohormones and their interactions in regulation of plant adaptation to numerous stressors. Intensive molecular studies have elucidated various plant hormonal pathways; each of which consist of many signaling components that link a specific hormone perception to the regulation of downstream genes. Signal transduction pathways of auxin, abscisic acid, cytokinins, gibberellins and ethylene have been thoroughly investigated. More recently, emerging signaling pathways of brassinosteroids, jasmonates, salicylic acid and strigolactones offer an exciting gateway for understanding their multiple roles in plant physiological processes.At the molecular level, phytohormonal crosstalks can be antagonistic or synergistic or additive in actions. Additionally, the signal transduction component(s) of one hormonal pathway may interplay with the signaling component(s) of other hormonal pathway(s). Together these and other research findings have revolutionized the concept of phytohormonal studies in plants. Importantly, genetic engineering now enables plant biologists to manipulate the signaling pathways of plant hormones for development of crop varieties with improved yield and stress tolerance.This book, written by internationally recognized scholars from various countries, represents the state-of-the-art understanding of plant hormones’ biology, signal transduction and implications. Aimed at a wide range of readers, including researchers, students, teachers and many others who have interests in this flourishing research field, every section is concluded with biotechnological strategies to modulate hormone contents or signal transduction pathways and crosstalk that enable us to develop crops in a sustainable manner. Given the important physiological implications of plant hormones in stressful environments, our book is finalized with chapters on phytohormonal crosstalks under abiotic and biotic stresses. } } @INBOOK{IPB-1575, author = {Vaira, A. M. and Gago-Zachert, S. and Garcia, M. L. and Guerri, J. and Hammond, J. and Milne, R. G. and Moreno, P. and Morikawa, T. and Natsuaki, T. and Navarro, J. A. and Pallas, V. and Torok, V. and Verbeek, M. and Vetten, H. J.}, title = {{Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses}}, year = {2012}, pages = {743-748}, chapter = {{Family - Ophioviridae}}, editor = {King, A. M. Q., et al., eds.}, doi = {10.1016/B978-0-12-384684-6.00060-4}, url = {https://dx.doi.org/10.1016/B978-0-12-384684-6.00060-4}, abstract = {This chapter focuses on Ophioviridae family whose sole member genus is Ophiovirus. The member species of the genus include Citrus psorosis virus (CPsV), Freesia sneak virus(FreSV), Lettuce ring necrosis virus (LRNV), and Mirafiori lettuce big-vein virus (MiLBVV).The single stranded negative/possibly ambisense RNA genome is divided into 3–4 segments, each of which is encapsidated in a single coat protein (43–50 kDa) forming filamentous virions of about 3 nm in diameter, in shape of kinked or probably internally coiled circles of at least two different contour lengths. Ophioviruses can be mechanically transmitted to a limited range of test plants, inducing local lesions and systemic mottle. The natural hosts of CPsV, ranunculus white mottle virus (RWMV), MiLBVV, and LRNV are dicotyledonous plants of widely differing taxonomy. CPsV has a wide geographical distribution in citrus in the Americas, in the Mediterranean and in New Zealand. FreSV has been reported in two species of the family Ranunculacae from Northern Italy, and in lettuce in France and Germany. Tulip mild mottle mosaic virus (TMMMV) has been reported in tulips in Japan. LRNV is closely associated with lettuce ring necrosis disease in The Netherlands, Belgium, and France, and FreSV has been reported in Europe, Africa, North America and New Zealand.} } @INBOOK{IPB-1499, author = {Dorka, R. and Miersch, O. and Hause, B. and Weik, P. and Wasternack, C.}, title = {{Die Mistel in der Tumortherapie 2. Aktueller Stand der Forschung und klinische Anwendung}}, year = {2009}, pages = {49-56}, chapter = {{Chronobiologische Phänomene und Jasmonatgehalt bei Viscum album L.}}, editor = {Scheer, R.; Bauer, R.; Bekker, A.; Berg, P. A.; Fintelmann, V.}, } @INBOOK{IPB-1567, author = {Flores, R. and Carbonell, A. and Gago, S. and Martínez de Alba, A.E. and Delgado, S. and Rodio, M.E. and di Serio, F.}, title = {{Biology of Plant-Microbe Interactions}}, year = {2008}, pages = {1-9}, chapter = {{Viroid-host interactions: A molecular dialogue between two uneven partners}}, editor = {Lorito, M., Woo, S. L., Scala, F.}, volume = {6 (chap. 58)}, } @Article{IPB-701, author = {Wasternack, C. and Stenzel, I. and Hause, B. and Hause, G. and Kutter, C. and Maucher, H. and Neumerkel, J. and Feussner, I. and Miersch, O.}, title = {{The wound response in tomato - Role of jasmonic acid}}, year = {2006}, pages = {297-306 }, journal = {J. Plant Physiol}, doi = {10.1016/j.jplph.2005.10.014}, volume = {163}, } @Article{IPB-699, author = {Sharma, V.K. and Monostori, T. and Hause, B. and Maucher, H. and Göbel, C. and Hornung, E. and Hänsch, R. and Bittner, F. and Wasternack, C. and Feussner, I. and Mendel, R.R. and Schulze, J.}, title = {{Genetic transformation of barley to modify expression of a 13-lipoxygenase}}, year = {2005}, pages = {33-34 }, journal = {Acta Biol. Szeged }, url = {http://www2.sci.u-szeged.hu/ABS/2005/Acta%20HP/4933.pdf}, volume = {49}, abstract = {Immature scutella of barley were transformed with cDNA coding for a 13-li-poxygenase of barley (LOX-100) via particle bombardment. Regenerated plants were tested by PAT-assay, Western-analysis and PCR-screening. Immunocytochemical assay of T0 plants showed expression of the LOX cDNA both in the chloroplasts and in the cytosol, depending on the presence of the chloroplast signal peptide sequences in the cDNA. A few transgenic plants containing higher amounts of LOX-derived products have been found. These are the candidates for further analysis concerning pathogen resistance.} } @INBOOK{IPB-457, author = {Wasternack, C. and Hause, B.}, title = {{Progress in Nucleic Acid Research and Molecular Biology}}, year = {2002}, pages = {165-221}, chapter = {{Jasmonates and octadecanoids: Signals in plant stress responses and development}}, editor = {Moldave, K.}, doi = {10.1016/S0079-6603(02)72070-9}, volume = {72}, }