TY - JOUR ID - 1725 TI - Simultaneous analysis of apolar phytohormones and 1-aminocyclopropan-1-carboxylic acid by high performance liquid chromatography/electrospray negative ion tandem mass spectrometry via 9-fluorenylmethoxycarbonyl chloride derivatization JO - J Chromatogr A PY - 2014 SP - 102-109 AU - Ziegler, J. AU - Qwegwer, J. AU - Schubert, M. AU - Erickson, J.L. AU - Schattat, M. AU - Bürstenbinder, K. AU - Grubb, C.D. AU - Abel, S. VL - 1362 UR - http://www.sciencedirect.com/science/article/pii/S002196731401259X DO - 10.1016/j.chroma.2014.08.029 AB - A strategy to detect and quantify the polar ethylene precursor 1-aminocyclopropan-1-carboxylic acid (ACC) along with the more apolar phytohormones abscisic acid (ABA), indole-3-acetic acid (IAA), jasmonic acid (JA), jasmonic acid-isoleucine conjugate (JA-Ile), 12-oxo-phytodienoic acid (OPDA), trans-zeatin, and trans-zeatin 9-riboside using a single extraction is presented. Solid phase resins commonly employed for extraction of phytohormones do not allow the recovery of ACC. We circumvent this problem by attaching an apolar group to ACC via derivatization with the amino group specific reagent 9-fluorenylmethoxycarbonyl chloride (Fmoc-Cl). Derivatization in the methanolic crude extract does not modify other phytohormones. The derivatized ACC could be purified and detected together with the more apolar phytohormones using common solid phase extraction resins and reverse phase HPLC/electrospray negative ion tandem mass spectrometry. The limit of detection was in the low nanomolar range for all phytohormones, a sensitivity sufficient to accurately determine the phytohormone levels from less than 50 mg (fresh weight) of Arabidopsis thaliana and Nicotiana benthamiana tissues. Comparison with previously published phytohormone levels and the reported changes in phytohormone levels after stress treatments confirmed the accuracy of the method. A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 699 TI - Genetic transformation of barley to modify expression of a 13-lipoxygenase JO - Acta Biol. Szeged PY - 2005 SP - 33-34 AU - Sharma, V.K. AU - Monostori, T. AU - Hause, B. AU - Maucher, H. AU - Göbel, C. AU - Hornung, E. AU - Hänsch, R. AU - Bittner, F. AU - Wasternack, C. AU - Feussner, I. AU - Mendel, R.R. AU - Schulze, J. VL - 49 UR - http://www2.sci.u-szeged.hu/ABS/2005/Acta%20HP/4933.pdf AB - Immature scutella of barley were transformed with cDNA coding for a 13-li-poxygenase of barley (LOX-100) via particle bombardment. Regenerated plants were tested by PAT-assay, Western-analysis and PCR-screening. Immunocytochemical assay of T0 plants showed expression of the LOX cDNA both in the chloroplasts and in the cytosol, depending on the presence of the chloroplast signal peptide sequences in the cDNA. A few transgenic plants containing higher amounts of LOX-derived products have been found. These are the candidates for further analysis concerning pathogen resistance. A2 - C1 - Molecular Signal Processing; Cell and Metabolic Biology ER - TY - JOUR ID - 355 TI - Activation of jasmonic acid production in Zea mays L. infected by the maize rough dwarf virus-Río Cuarto. Reversion of symptoms by salicylic acid JO - Biocell PY - 2002 SP - 369-374 AU - Vigliocco, A. AU - Bonamico, M.B. AU - Alemano, S. AU - Miersch, O. AU - Abdala, G. VL - 26(3) UR - AB - A2 - C1 - Molecular Signal Processing ER -