TY - JOUR ID - 699 TI - Genetic transformation of barley to modify expression of a 13-lipoxygenase JO - Acta Biol. Szeged PY - 2005 SP - 33-34 AU - Sharma, V.K. AU - Monostori, T. AU - Hause, B. AU - Maucher, H. AU - Göbel, C. AU - Hornung, E. AU - Hänsch, R. AU - Bittner, F. AU - Wasternack, C. AU - Feussner, I. AU - Mendel, R.R. AU - Schulze, J. VL - 49 UR - http://www2.sci.u-szeged.hu/ABS/2005/Acta%20HP/4933.pdf AB - Immature scutella of barley were transformed with cDNA coding for a 13-li-poxygenase of barley (LOX-100) via particle bombardment. Regenerated plants were tested by PAT-assay, Western-analysis and PCR-screening. Immunocytochemical assay of T0 plants showed expression of the LOX cDNA both in the chloroplasts and in the cytosol, depending on the presence of the chloroplast signal peptide sequences in the cDNA. A few transgenic plants containing higher amounts of LOX-derived products have been found. These are the candidates for further analysis concerning pathogen resistance. A2 - C1 - Molecular Signal Processing; Cell and Metabolic Biology ER -