TY - JOUR ID - 2390 TI - Molecular characterization and evolution studies of a SERK like gene transcriptionally induced during somatic embryogenesis in Phoenix Dactylifera L v Deglet Nour JO - Genetika PY - 2015 SP - 323-337 AU - Rekik, I. AU - Drira, N. AU - Grubb, C. D. AU - Elleuch, A. VL - 47 UR - https://dx.doi.org/10.2298/GENSR1501323R DO - 10.2298/GENSR1501323R AB - A somatic embryogenesis receptor kinase like (SERKL) cDNA, designated PhSERKL, was isolated from date palm (Phoenix Dactylifera L) using RACE PCR. PhSERKL protein shared all the characteristic domains of the SERK family, including five leucine-rich repeats, one proline-rich region motif, a transmembrane domain, and kinase domains. Phylogenetic analyses using PHYLIP and Notung 2.7 programs suggest that the SERK proteins of some plant species resulted from relatively ancient duplication events. We predict an ancestor protein of monocots and dicots SERK using FASTML program. Somatic embryogenic cultures of date palm were established following transfer of callus cultures to medium containing 2, 4-dichlorophenoxyacetic acid. The role of PhSERKL gene during establishment of somatic embryogenesis in culture was investigated using quantitative real-time PCR. PhSERKL gene was highly expressed during embryogenic competence acquisition and globular embryo formation in culture. Overall, levels of expression of PhSERKL gene were lower in nonembryogenic tissues and organs than in embryogenic callus. A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 2386 TI - First report of Citrus viroid V in North Africa JO - J Gen Plant Pathol PY - 2015 SP - 87-91 AU - Hamdi, I. AU - Elleuch, A. AU - Bessaies, N. AU - Grubb, C. D. AU - Fakhfakh, H. VL - 81 UR - https://dx.doi.org/10.1007/s10327-014-0556-9 DO - 10.1007/s10327-014-0556-9 AB - We tested citrus samples from Tunisia using reverse transcription-polymerase chain reaction (RT-PCR), and for the first time, Citrus viroid V (CVd-V) was reported in North Africa. Fourteen of 38 tested citrus trees were infected by CVd-V including the majority of varieties grown in Tunisia. Some RT-PCR results were also supported by biological indexing. After sequencing the RT-PCR products, three new CVd-V variants were identified, showing 80–91 % nucleotide sequence identity with those reported previously. Based on phylogenetic analysis using all CVd-V sequences in GenBank, two main CVd-V groups were identified. Furthermore, construction of a genetic network of the detected haplotypes using the same sequences shows a clear geographical structuring of Tunisian CVd-V variants. A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 1147 TI - Inheritance of three major genes involved in the synthesis of aliphatic glucosinolates in Brassica oleracea JO - J Amer Soc Hort Sci PY - 2001 SP - 427 - 431 AU - Li, G. AU - Goyal, G.S. AU - Abel, S. AU - Quiros, C.F. VL - 126 UR - AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 862 TI - Breeding for virus resistance in maize JO - Genetika PY - 2000 SP - 283-291 AU - Quint, M. AU - Melchinger, A.E. AU - Dussle, C.M. AU - Lübberstedt, T. VL - 32 UR - AB - A2 - C1 - Molecular Signal Processing ER -