TY - JOUR ID - 2390 TI - Molecular characterization and evolution studies of a SERK like gene transcriptionally induced during somatic embryogenesis in Phoenix Dactylifera L v Deglet Nour JO - Genetika PY - 2015 SP - 323-337 AU - Rekik, I. AU - Drira, N. AU - Grubb, C. D. AU - Elleuch, A. VL - 47 UR - https://dx.doi.org/10.2298/GENSR1501323R DO - 10.2298/GENSR1501323R AB - A somatic embryogenesis receptor kinase like (SERKL) cDNA, designated PhSERKL, was isolated from date palm (Phoenix Dactylifera L) using RACE PCR. PhSERKL protein shared all the characteristic domains of the SERK family, including five leucine-rich repeats, one proline-rich region motif, a transmembrane domain, and kinase domains. Phylogenetic analyses using PHYLIP and Notung 2.7 programs suggest that the SERK proteins of some plant species resulted from relatively ancient duplication events. We predict an ancestor protein of monocots and dicots SERK using FASTML program. Somatic embryogenic cultures of date palm were established following transfer of callus cultures to medium containing 2, 4-dichlorophenoxyacetic acid. The role of PhSERKL gene during establishment of somatic embryogenesis in culture was investigated using quantitative real-time PCR. PhSERKL gene was highly expressed during embryogenic competence acquisition and globular embryo formation in culture. Overall, levels of expression of PhSERKL gene were lower in nonembryogenic tissues and organs than in embryogenic callus. A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 2386 TI - First report of Citrus viroid V in North Africa JO - J Gen Plant Pathol PY - 2015 SP - 87-91 AU - Hamdi, I. AU - Elleuch, A. AU - Bessaies, N. AU - Grubb, C. D. AU - Fakhfakh, H. VL - 81 UR - https://dx.doi.org/10.1007/s10327-014-0556-9 DO - 10.1007/s10327-014-0556-9 AB - We tested citrus samples from Tunisia using reverse transcription-polymerase chain reaction (RT-PCR), and for the first time, Citrus viroid V (CVd-V) was reported in North Africa. Fourteen of 38 tested citrus trees were infected by CVd-V including the majority of varieties grown in Tunisia. Some RT-PCR results were also supported by biological indexing. After sequencing the RT-PCR products, three new CVd-V variants were identified, showing 80–91 % nucleotide sequence identity with those reported previously. Based on phylogenetic analysis using all CVd-V sequences in GenBank, two main CVd-V groups were identified. Furthermore, construction of a genetic network of the detected haplotypes using the same sequences shows a clear geographical structuring of Tunisian CVd-V variants. A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 1650 TI - UHPLC-MS/MS based target profiling of stress-induced phytohormones JO - Phytochemistry PY - 2014 SP - 147-157 AU - Floková, K. AU - Tarkowská, D. AU - Miersch, O. AU - Strnad, M. AU - Wasternack, C. AU - Novak, O. VL - 105 UR - http://www.sciencedirect.com/science/journal/00319422 DO - 10.1016/j.phytochem.2014.05.015 AB - Stress-induced changes in phytohormone metabolite profiles have rapid effects on plant metabolic activity and growth. The jasmonates (JAs) are a group of fatty acid-derived stress response regulators with roles in numerous developmental processes. To elucidate their dual regulatory effects, which overlap with those of other important defence-signalling plant hormones such as salicylic acid (SA), abscisic acid (ABA) and indole-3-acetic acid (IAA), we have developed a highly efficient single-step clean-up procedure for their enrichment from complex plant matrices that enables their sensitive quantitative analysis using hyphenated mass spectrometry technique. The rapid extraction of minute quantities of plant material (less than 20 mg fresh weight, FW) into cold 10% methanol followed by one-step reversed-phase polymer-based solid phase extraction significantly reduced matrix effects and increased the recovery of labile JA analytes. This extraction and purification protocol was paired with a highly sensitive and validated ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method and used to simultaneously profile sixteen stress-induced phytohormones in minute plant material samples, including endogenous JA, several of its biosynthetic precursors and derivatives, as well as SA, ABA and IAA. A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 1305 TI - Chemoenzymatic synthesis of diverse thiohydroximates from glucosinolate-utilizing enzymes from Helix pomatia and Caldicellulosiruptor saccharolyticus JO - Biotechnol Lett PY - 2011 SP - 1039-1046 AU - Kopycki, J. AU - Schmidt, J. AU - Abel, S. AU - Grubb, C. D. VL - 33 UR - http://www.springerlink.com/content/p4x00m77787534t6/fulltext.pdf DO - 10.1007/s10529-011-0530-y AB - Thiohydroximates comprise a diverse class of compounds important in both biological and industrial chemistry. Their syntheses are generally limited to simple alkyl and aryl compounds with few stereocenters and a narrow range of functional groups. We hypothesized that sequential action of two recombinant enzymes, a sulfatase from Helix pomatia and a β-O-glucosidase from Caldicellulosiruptor saccharolyticus, on glucosinolates would allow synthesis of thiohydroximates from a structurally broad array of abundant precursors. We report successful synthesis of thiohydroximates of varied chemical classes, including from homochiral compounds of demonstrated biological activity. The chemoenzymatic synthetic route reported here should allow access to many, if not all, of the thiohydroximate core structures of the ~200 known naturally occurring glucosinolates. The enrichment of this group for compounds with possible pharmacological potential is discussed. A2 - C1 - Molecular Signal Processing; Bioorganic Chemistry ER - TY - JOUR ID - 1147 TI - Inheritance of three major genes involved in the synthesis of aliphatic glucosinolates in Brassica oleracea JO - J Amer Soc Hort Sci PY - 2001 SP - 427 - 431 AU - Li, G. AU - Goyal, G.S. AU - Abel, S. AU - Quiros, C.F. VL - 126 UR - AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 862 TI - Breeding for virus resistance in maize JO - Genetika PY - 2000 SP - 283-291 AU - Quint, M. AU - Melchinger, A.E. AU - Dussle, C.M. AU - Lübberstedt, T. VL - 32 UR - AB - A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 388 TI - Induction of a new lipoxygenase form in cucumber leaves by salicylic acid or 2,6-dichloroisonicotinic acid JO - Bot. Acta PY - 1997 SP - 101-108 AU - Feussner, I. AU - Fritz, I.G. AU - Hause, B. AU - Ullrich, W.R. AU - Wasternack, C. VL - 110 UR - http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1438-8677/issues DO - 10.1111/j.1438-8677.1997.tb00616.x AB - Changes in lipoxygenase (LOX) protein pattern and/or activity were investigated in relation to acquired resistance of cucumber (Cucumis sativus L.) leaves against two powdery mildews, Sphaerotheca fuliginea (Schlecht) Salmon and Erysiphe cichoracearum DC et Merat. Acquired resistance was established by spraying leaves with salicylic acid (SA) or 2,6-dichloroisonicotinic acid (INA) and estimated in whole plants by infested leaf area compared to control plants. SA was more effective than INA. According to Western blots, untreated cucumber leaves contained a 97 kDa LOX form, which remained unchanged for up to 48 h after pathogen inoculation. Upon treatment with SA alone for 24 h or with INA plus pathogen, an additional 95 kDa LOX form appeared which had an isoelectric point in the alkaline range. For the induction of this form, a threshold concentration of 1 mM SA was required, higher SA concentrations did not change LOX-95 expression which remained similar between 24 h and 96 h but further increased upon mildew inoculation. Phloem exudates contained only the LOX-97 form, in intercellular washing fluid no LOX was detected. dichloroisonicotinic localization revealed LOX protein in the cytosol of the mesophyll cells without differences between the forms. A2 - C1 - Molecular Signal Processing; Cell and Metabolic Biology ER -