TY - JOUR ID - 1650 TI - UHPLC-MS/MS based target profiling of stress-induced phytohormones JO - Phytochemistry PY - 2014 SP - 147-157 AU - Floková, K. AU - Tarkowská, D. AU - Miersch, O. AU - Strnad, M. AU - Wasternack, C. AU - Novak, O. VL - 105 UR - http://www.sciencedirect.com/science/journal/00319422 DO - 10.1016/j.phytochem.2014.05.015 AB - Stress-induced changes in phytohormone metabolite profiles have rapid effects on plant metabolic activity and growth. The jasmonates (JAs) are a group of fatty acid-derived stress response regulators with roles in numerous developmental processes. To elucidate their dual regulatory effects, which overlap with those of other important defence-signalling plant hormones such as salicylic acid (SA), abscisic acid (ABA) and indole-3-acetic acid (IAA), we have developed a highly efficient single-step clean-up procedure for their enrichment from complex plant matrices that enables their sensitive quantitative analysis using hyphenated mass spectrometry technique. The rapid extraction of minute quantities of plant material (less than 20 mg fresh weight, FW) into cold 10% methanol followed by one-step reversed-phase polymer-based solid phase extraction significantly reduced matrix effects and increased the recovery of labile JA analytes. This extraction and purification protocol was paired with a highly sensitive and validated ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method and used to simultaneously profile sixteen stress-induced phytohormones in minute plant material samples, including endogenous JA, several of its biosynthetic precursors and derivatives, as well as SA, ABA and IAA. A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 701 TI - The wound response in tomato - Role of jasmonic acid JO - J. Plant Physiol PY - 2006 SP - 297-306 AU - Wasternack, C. AU - Stenzel, I. AU - Hause, B. AU - Hause, G. AU - Kutter, C. AU - Maucher, H. AU - Neumerkel, J. AU - Feussner, I. AU - Miersch, O. VL - 163 UR - DO - 10.1016/j.jplph.2005.10.014 AB - A2 - C1 - Molecular Signal Processing; Cell and Metabolic Biology ER -