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Publikationen - Molekulare Signalverarbeitung

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Publikation

Krägeloh, T.; Cavalleri, J. M. V.; Ziegler, J.; Sander, J.; Terhardt, M.; Breves, G.; Cehak, A. Identification of hypoglycin A binding adsorbents as potential preventive measures in co-grazers of atypical myopathy affected horses Equine Vet J 50, 220-227, (2018) DOI: 10.1111/evj.12723

BackgroundIntestinal absorption of hypoglycin A (HGA) and its metabolism are considered major prerequisites for atypical myopathy (AM). The increasing incidence and the high mortality rate of AM urgently necessitate new therapeutic and/or preventative approaches.ObjectivesTo identify a substance for oral administration capable of binding HGA in the intestinal lumen and effectively reducing the intestinal absorption of the toxin.Study designExperimental in vitro study.MethodsSubstances commonly used in equine practice (activated charcoal composition, di‐tri‐octahedral smectite, mineral oil and activated charcoal) were tested for their binding capacity for HGA using an in vitro incubation method. The substance most effective in binding HGA was subsequently tested for its potential to reduce intestinal HGA absorption. Jejunal tissues of 6 horses were incubated in Ussing chambers to determine mucosal uptake, tissue accumulation, and serosal release of HGA in the presence and absence of the target substance. Potential intestinal metabolism in methylenecyclopropyl acetic acid (MCPA)‐conjugates was investigated by analysing their concentrations in samples from the Ussing chambers.ResultsActivated charcoal composition and activated charcoal were identified as potent HGA binding substances with dose and pH dependent binding capacity. There was no evidence of intestinal HGA metabolism.Main limitationsBinding capacity of adsorbents was tested in vitro using aqueous solutions, and in vivo factors such as transit time and composition of intestinal content, may affect adsorption capacity after oral administration.ConclusionsFor the first time, this study identifies substances capable of reducing HGA intestinal absorption. This might have major implications as a preventive measure in cograzers of AM affected horses but also in horses at an early stage of intoxication.
Publikation

Schilling, S.; Stenzel, I.; von Bohlen, A.; Wermann, M.; Schulz, K.; Demuth, H.-U.; Wasternack, C. Isolation and characterization of the glutaminyl cyclases from <i>Solanum tuberosum</i> and <i>Arabidopsis thaliana</i>: implications for physiological functions Biol. Chem 388, 145-153, (2007)

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Publikation

Quint, M.; Gray, W.M. Auxin signaling Curr Opin Plant Biol 9, 448-453, (2006)

Auxin regulates a host of plant developmental and physiological processes, including embryogenesis, vascular differentiation, organogenesis, tropic growth, and root and shoot architecture. Genetic and biochemical studies carried out over the past decade have revealed that much of this regulation involves the SCFTIR1/AFB-mediated proteolysis of the Aux/IAA family of transcriptional regulators. With the recent finding that the TRANSPORT INHIBITOR RESPONSE1 (TIR1)/AUXIN SIGNALING F-BOX (AFB) proteins also function as auxin receptors, a potentially complete, and surprisingly simple, signaling pathway from perception to transcriptional response is now before us. However, understanding how this seemingly simple pathway controls the myriad of specific auxin responses remains a daunting challenge, and compelling evidence exists for SCFTIR1/AFB-independent auxin signaling pathways.
Bücher und Buchkapitel

Stumpe, M.; Stenzel, I.; Weichert, H.; Hause, B.; Feussner, I. The lipoxygenase pathway in mycorrhizal roots of <span>Medicago truncatula</span> (Murata, N., Yamada, M., Nishida, I., Okuyama, H., Sekijar, J., Hajme, W.). Kluwer Academic Publishers, Dordrecht 287-290, (2003)

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Publikation

Gago, S.; Costa, N.; Semorile, L.; Grau, O. Sequence variability in p27 gene of Citrus tristeza virus (CTV) revealed by SSCP analysis Electronic Journal of Biotechnology 2, 41-50, (1999)

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Bücher und Buchkapitel

Feussner, I.; Kühn, H.; Wasternack, C. Do Lipoxygenases initiate ß-oxidation? (Williams, J.P., Mobashsher, U., Khan, M.U. & Lem, N.W.). Kluwer Academic Publishers, Dordrecht 250-252, (1997)

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Bücher und Buchkapitel

Ziegler, J.; Hamberg, M.; Miersch, O. Allene oxide cyclase from corn: Partial purification and characterization (Williams, J.P., Mobashsher, U., Khan, M.U., Lem, N.W.). Kluwer Academic Publishers, Dordrecht 99-101, (1997)

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