zur Suche springenzur Navigation springenzum Inhalt springen

Publikationen - Molekulare Signalverarbeitung

Sortieren nach: Erscheinungsjahr Typ der Publikation

Zeige Ergebnisse 1 bis 4 von 4.

Publikation

Ryan,P. T.; Ó’Maoiléidigh, D. S.; Drost, H.-G.; Kwaśniewska, D.; Gabel, A.; Grosse, I.; Graciet, E.; Quint, M.; Wellmer, F. Patterns of gene expression during Arabidopsis flower development from the time of initiation to maturation BMC Genomics 16, 488 , (2015) DOI: 10.1186/s12864-015-1699-6

Background:The formation of flowers is one of the main model systems to elucidate the molecular mechanisms that control developmental processes in plants. Although several studies have explored gene expression during flower development in the model plant Arabidopsis thalianaon a genome-wide scale, a continuous series of expression data from the earliest floral stages until maturation has been lacking. Here, we used a floral induction system to closethis information gap and to generate a reference dataset for stage-specific gene expression during flower formation.Results:Using a floral induction system, we collected floral buds at 14 different stages from the time of initiation until maturation. Using whole-genome microarray analysis, we identified 7,405 genes that exhibit rapid expression changes during flower development. These genes comprise many known floral regulators and we found that the expression profiles for these regulators match their known expression patterns, thus validating the dataset. We analyzed groups ofco-expressed genes for over-represented cellular and developmental functions through Gene Ontology analysis and found that they could be assigned specific patterns of activities, which are in agreement with the progression of flower development. Furthermore, by mapping binding sites of floral organ identity factors onto our dataset, we were able to identify gene groups that are likely predominantly under control of these transcriptional regulators. We furtherfound that the distribution of paralogs among groups of co-expressed genes varies considerably, with genes expressed predominantly at early and intermediate stages of flower development showing the highest proportion of such genes.Conclusions:Our results highlight and describe the dynamic expression changes undergone by a large numberof genes during flower development. They further provide a comprehensive reference dataset for temporal gene expression during flower formation and we demonstrate that it can be used to integrate data from other genomics approaches such as genome-wide localization studies of transcription factor binding sites.
Publikation

Wasternack, C.; Hause, B. Jasmonates: biosynthesis, perception, signal transduction and action in plant stress response, growth and development. An update to the 2007 review in <span>Annals of Botany</span> Annals of Botany 111, 1021-1058, (2013) DOI: 10.1093/aob/mct067

Background: Jasmonates are important regulators in plant responses to biotic and abiotic stresses as well as indevelopment. Synthesized from lipid-constituents, the initially formed jasmonic acid is converted to differentmetabolites including the conjugate with isoleucine. Important new components of jasmonate signalling includingits receptor were identified, providing deeper insight into the role of jasmonate signalling pathways in stressresponses and development.Scope: The present review is an update of the review on jasmonates published in this journal in 2007. New dataof the last five years are described with emphasis on metabolites of jasmonates, on jasmonate perception andsignalling, on cross-talk to other plant hormones and on jasmonate signalling in response to herbivores and pathogens,in symbiotic interactions, in flower development, in root growth and in light perception.Conclusions: The last few years have seen breakthroughs in the identification of JASMONATE ZIM DOMAIN(JAZ) proteins and their interactors such as transcription factors and co-repressors, and the crystallization of thejasmonate receptor as well as of the enzyme conjugating jasmonate to amino acids. Now, the complex nature ofnetworks of jasmonate signalling in stress responses and development including hormone cross-talk can beaddressed.
Publikation

Schilling, S.; Stenzel, I.; von Bohlen, A.; Wermann, M.; Schulz, K.; Demuth, H.-U.; Wasternack, C. Isolation and characterization of the glutaminyl cyclases from <i>Solanum tuberosum</i> and <i>Arabidopsis thaliana</i>: implications for physiological functions Biol. Chem 388, 145-153, (2007)

0
Publikation

Abel, S.; Ballas, N.; Wong, L-M.; Theologis, A. DNA elements responsive to auxin Bio Essays 18(8), 647-654, (1996)

Genes induced by the plant hormone auxin are probably involved in the execution of vital cellular functions and developmental processes. Experimental approaches designed to elucidate the molecular mechanisms of auxin action have focused on auxin perception, genetic dissection of the signaling apparatus and specific gene activation. Auxin-responsive promoter elements of early genes provide molecular tools for probing auxin signaling in reverse. Functional analysis of several auxin-specific promoters of unrelated early genes suggests combinatorial utilization of both conserved and variable elements. These elements are arranged into autonomous domains and the combination of such modules generates uniquely composed promoters. Modular promoters allow for auxin-mediated transcriptional responses to be revealed in a tissue- and development-specific manner.
IPB Mainnav Search