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Journal / Buchreihe / Preprint-Server Nach Häufigkeit alphabetisch sortiert: Biol. Chem
Journal / Buchreihe / Preprint-Server Nach Häufigkeit alphabetisch sortiert: ACS Chem. Biol doi: 10.1021/cb900269u
Journal / Buchreihe / Preprint-Server Nach Häufigkeit alphabetisch sortiert: J Gen Plant Pathol
Journal / Buchreihe / Preprint-Server Nach Häufigkeit alphabetisch sortiert: Biotechnol Lett
Journal / Buchreihe / Preprint-Server Nach Häufigkeit alphabetisch sortiert: Comprehensive Natural Products II
Journal / Buchreihe / Preprint-Server Nach Häufigkeit alphabetisch sortiert: Journal of Plant Growth Regulation
Journal / Buchreihe / Preprint-Server Nach Häufigkeit alphabetisch sortiert: Cereal Res. Commun.
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Monostori, T.; Schulze, J.; Sharma, V. K.; Maucher, H.; Wasternack, C.; Hause, B.; Novel plasmid vectors for homologous transformation of barley (Hordeum vulgare L.) with JIP23 cDNA in sense and antisense orientation Cereal Res. Commun. 31, 17-24, (2003) DOI: 10.1007/BF03543245
The most abundant jasmonate-induced protein (JIP) in barley leaves is a 23 kDa protein (JIP23). Its function, however, is unknown. In order to analyze its function by homologous transformation, new plasmid vectors have been constructed. They carry the cDNA coding for JIP23 in sense or antisense orientation under the control of the Ubi-1-promoter as well as the pat resistance gene under the control of the 35S promoter. Barley mesophyll protoplasts were transiently transformed with the sense constructs. PAT activity and immunological detection of JIP23 could be achieved in transformed protoplasts but not in untransformed protoplasts indicating that the construct was active. Thus, these new vectors are suitable for stable transformation of barley. Carrying a multiple cloning site (MCS), these vectors can be used now in a wide range of transformation of barley.