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Publikation

Hamdi, I.; Elleuch, A.; Bessaies, N.; Grubb, C. D.; Fakhfakh, H. First report of Citrus viroid V in North Africa J Gen Plant Pathol 81, 87-91, (2015) DOI: 10.1007/s10327-014-0556-9

We tested citrus samples from Tunisia using reverse transcription-polymerase chain reaction (RT-PCR), and for the first time, Citrus viroid V (CVd-V) was reported in North Africa. Fourteen of 38 tested citrus trees were infected by CVd-V including the majority of varieties grown in Tunisia. Some RT-PCR results were also supported by biological indexing. After sequencing the RT-PCR products, three new CVd-V variants were identified, showing 80–91 % nucleotide sequence identity with those reported previously. Based on phylogenetic analysis using all CVd-V sequences in GenBank, two main CVd-V groups were identified. Furthermore, construction of a genetic network of the detected haplotypes using the same sequences shows a clear geographical structuring of Tunisian CVd-V variants.
Publikation

Kopycki, J.; Schmidt, J.; Abel, S.; Grubb, C. D. Chemoenzymatic synthesis of diverse thiohydroximates from glucosinolate-utilizing enzymes from Helix pomatia and Caldicellulosiruptor saccharolyticus Biotechnol Lett 33, 1039-1046, (2011) DOI: 10.1007/s10529-011-0530-y

Thiohydroximates comprise a diverse class of compounds important in both biological and industrial chemistry. Their syntheses are generally limited to simple alkyl and aryl compounds with few stereocenters and a narrow range of functional groups. We hypothesized that sequential action of two recombinant enzymes, a sulfatase from Helix pomatia and a β-O-glucosidase from Caldicellulosiruptor saccharolyticus, on glucosinolates would allow synthesis of thiohydroximates from a structurally broad array of abundant precursors. We report successful synthesis of thiohydroximates of varied chemical classes, including from homochiral compounds of demonstrated biological activity. The chemoenzymatic synthetic route reported here should allow access to many, if not all, of the thiohydroximate core structures of the ~200 known naturally occurring glucosinolates. The enrichment of this group for compounds with possible pharmacological potential is discussed.
Bücher und Buchkapitel

Yamaguchi, I.; Cohen, J. D.; Culler, A. H.; Quint, M.; Slovin, J. P.; Nakajima, M.; Yamaguchi, S.; Sakakibara, H.; Kuroha, T.; Hirai, N.; Yokota, T.; Ohta, H.; Kobayashi, Y.; Mori, H.; Sakagami, Y. Plant Hormones (Liu, H.-W. & Mander, L., eds.). Comprehensive Natural Products II 4, 9-125, (2010) ISBN: 978-0-08-045382-8 DOI: 10.1016/B978-008045382-8.00092-7

The definition of a plant hormone has not been clearly established, so the compounds classified as plant hormones often vary depending on which definition is considered. In this chapter, auxins, gibberellins (GAs), cytokinins, abscisic acid, brassinosteroids, jasmonic acid-related compounds, and ethylene are described as established plant hormones, while polyamines and phenolic compounds are not included. On the other hand, several peptides that have been proven to play a clear physiological role(s) in plant growth and development, similar to the established plant hormones, are referred. This chapter will focus primarily on the more recent discoveries of plant hormones and their impact on our current understanding of their biological role. In some cases, however, it is critical to place recent work in a proper historical context.
Publikation

Schilling, S.; Stenzel, I.; von Bohlen, A.; Wermann, M.; Schulz, K.; Demuth, H.-U.; Wasternack, C. Isolation and characterization of the glutaminyl cyclases from Solanum tuberosum and Arabidopsis thaliana: implications for physiological functions Biol. Chem 388, 145-153, (2007) DOI: 10.1515/BC.2007.016

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