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Publikationen - Molekulare Signalverarbeitung

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Publikation

Jayaweera, T.; Siriwardana, C.; Dharmasiri, S.; Quint, M.; Gray, W. M.; Dharmasiri, N. Alternative Splicing of Arabidopsis IBR5 Pre-mRNA Generates Two IBR5 Isoforms with Distinct and Overlapping Functions PLoS ONE 9, e102301, (2014) DOI: 10.1371/journal.pone.0102301

The INDOLE-3-BUTYRIC ACID RESPONSE5 (IBR5) gene encodes a dual specificity phosphatase that regulates plant auxinresponses. IBR5 has been predicted to generate two transcripts through alternative splicing, but alternative splicing of IBR5has not been confirmed experimentally. The previously characterized ibr5-1 null mutant exhibits many auxin related defectssuch as auxin insensitive primary root growth, defective vascular development, short stature and reduced lateral rootdevelopment. However, whether all these defects are caused by the lack of phosphatase activity is not clear. Here wedescribe two new auxin insensitive IBR5 alleles, ibr5-4, a catalytic site mutant, and ibr5-5, a splice site mutant.Characterization of these new mutants indicates that IBR5 is post-transcriptionally regulated to generate two transcripts,AT2G04550.1 and AT2G04550.3, and consequently two IBR5 isoforms, IBR5.1 and IBR5.3. The IBR5.1 isoform exhibitsphosphatase catalytic activity that is required for both proper degradation of Aux/IAA proteins and auxin-induced geneexpression. These two processes are independently regulated by IBR5.1. Comparison of new mutant alleles with ibr5-1indicates that all three mutant alleles share many phenotypes. However, each allele also confers distinct defects implicatingIBR5 isoform specific functions. Some of these functions are independent of IBR5.1 catalytic activity. Additionally, analysis ofthese new mutant alleles suggests that IBR5 may link ABP1 and SCFTIR1/AFBs auxin signaling pathways.
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