Ibañez, C.; Poeschl, Y.; Peterson, T.; Bellstädt, J.; Denk, K.; Gogol-Döring, A.; Quint, M.; Delker, C. Ambient temperature and genotype differentially affect developmental and phenotypic plasticity in Arabidopsis thaliana BMC Plant Biol 17, 114, (2017) DOI: 10.1186/s12870-017-1068-5
BackgroundGlobal increase in ambient temperatures
constitute a significant challenge to wild and cultivated plant species.
Forward genetic analyses of individual temperature-responsive traits
have resulted in the identification of several signaling and response
components. However, a comprehensive knowledge about temperature
sensitivity of different developmental stages and the contribution of
natural variation is still scarce and fragmented at best.ResultsHere, we
systematically analyze thermomorphogenesis throughout a complete life
cycle in ten natural Arabidopsis thaliana accessions grown under long
day conditions in four different temperatures ranging from 16 to 28 °C.
We used Q10, GxE, phenotypic divergence and correlation analyses to
assess temperature sensitivity and genotype effects of more than 30
morphometric and developmental traits representing five phenotype
classes. We found that genotype and temperature differentially affected
plant growth and development with variing strengths. Furthermore,
overall correlations among phenotypic temperature responses was
relatively low which seems to be caused by differential capacities for
temperature adaptations of individual
accessions.ConclusionGenotype-specific temperature responses may be
attractive targets for future forward genetic approaches and
accession-specific thermomorphogenesis maps may aid the assessment of
functional relevance of known and novel regulatory components.
Bürstenbinder, K.; Mitra, D.; Quegwer, J. Functions of IQD proteins as hubs in cellular calcium and auxin signaling: a toolbox for shape formation and tissue-specification in plants? Plant Signal Behav 12 , e1331198, (2017) DOI: 10.1080/15592324.2017.1331198
Ca2+ ions play pivotal roles as second messengers in intracellular signal transduction, and coordinate many biological processes. Changes in intracellular Ca2+ levels are perceived by Ca2+ sensors such as CaM/CML proteins, which transduce Ca2+ signals into cellular responses by regulation of diverse target proteins. Insights into molecular functions of CaM targets are thus essential to understand the molecular and cellular basis of Ca2+ signaling. During the last decade, IQD proteins emerged as the largest class of CaM targets in plants with mostly unknown functions. In the March issue of Plant Physiology, we presented the first comprehensive characterization of the 33-membered IQD family in Arabidopsis thaliana. We showed, by analysis of the subcellular localization of translational GFP fusion proteins, that most IQD members label MTs, and additionally often localize to the cell nucleus or to membranes, where the recruit CaM Ca2+ sensors. Important functions at MTs are supported by altered MT organization and plant growth in IQD gain-of-function lines. Because IQD proteins share structural hallmarks of scaffold proteins, we propose roles of IQDs in the assembly of macromolecular complexes to orchestrate Ca2+ CaM signaling from membranes to the nucleus.