Ibañez, C.; Poeschl, Y.; Peterson, T.; Bellstädt, J.; Denk, K.; Gogol-Döring, A.; Quint, M.; Delker, C. Ambient temperature and genotype differentially affect developmental and phenotypic plasticity in Arabidopsis thaliana BMC Plant Biol 17, 114, (2017) DOI: 10.1186/s12870-017-1068-5
BackgroundGlobal increase in ambient temperatures
constitute a significant challenge to wild and cultivated plant species.
Forward genetic analyses of individual temperature-responsive traits
have resulted in the identification of several signaling and response
components. However, a comprehensive knowledge about temperature
sensitivity of different developmental stages and the contribution of
natural variation is still scarce and fragmented at best.ResultsHere, we
systematically analyze thermomorphogenesis throughout a complete life
cycle in ten natural Arabidopsis thaliana accessions grown under long
day conditions in four different temperatures ranging from 16 to 28 °C.
We used Q10, GxE, phenotypic divergence and correlation analyses to
assess temperature sensitivity and genotype effects of more than 30
morphometric and developmental traits representing five phenotype
classes. We found that genotype and temperature differentially affected
plant growth and development with variing strengths. Furthermore,
overall correlations among phenotypic temperature responses was
relatively low which seems to be caused by differential capacities for
temperature adaptations of individual
accessions.ConclusionGenotype-specific temperature responses may be
attractive targets for future forward genetic approaches and
accession-specific thermomorphogenesis maps may aid the assessment of
functional relevance of known and novel regulatory components.
Liu, S.; Ziegler, J.; Zeier, J.; Birkenbihl, R. P.; Somssich, I. E. Botrytis cinerea B05.10 promotes disease development in Arabidopsis by suppressing WRKY33-mediated host immunity Plant Cell Environ 40, 2189-2206, (2017) DOI: 10.1111/pce.13022
The large WRKY transcription factor family is mainly involved in regulating plant immune responses. Arabidopsis WRKY33 is a key transcriptional regulator of hormonal and metabolic processes towards Botrytis cinerea strain 2100 infection and is essential for resistance. In contrast to B. cinerea strain 2100, the strain B05.10 is virulent on wild-type (WT) Col-0 Arabidopsis plants highlighting the genetic diversity within this pathogen species. We analysed how early WRKY33-dependent responses are affected upon infection with strain B05.10 and found that most of these responses were strongly dampened during this interaction. Ectopic expression of WRKY33 resulted in complete resistance towards this strain indicating that virulence of B05.10, at least partly, depends on suppressing WRKY33 expression/protein accumulation. As a consequence, the expression levels of direct WRKY33 target genes, including those involved in the biosynthesis of camalexin, were also reduced upon infection. Concomitantly, elevated levels of the phytohormone abscisic acid (ABA) were observed. Molecular and genetic studies revealed that ABA negatively influences defence to B05.10 and effects jasmonic acid/ethylene (JA/ET) and salicylic acid (SA) levels. Susceptibility/resistance was determined by the antagonistic effect of ABA on JA, and this crosstalk required suppressing WRKY33 functions at early infection stages. This indicates that B. cinerea B05.10 promotes disease by suppressing WRKY33-mediated host defences.