Publikationen - Molekulare Signalverarbeitung
Aktive Filter
Journal / Buchreihe / Preprint-Server Nach Häufigkeit alphabetisch sortiert: Acta Biol. Szeged
Journal / Buchreihe / Preprint-Server Nach Häufigkeit alphabetisch sortiert: Environ Exp Bot
Journal / Buchreihe / Preprint-Server Nach Häufigkeit alphabetisch sortiert: J. Plant Physiol
Journal / Buchreihe / Preprint-Server Nach Häufigkeit alphabetisch sortiert: 0
Typ der Publikation: Bücher und Buchkapitel
Erscheinungsjahr: 2019
Alle Filter entfernen
Suchfilter
Zeige Ergebnisse 1 bis 1 von 1.
Möller, B.; Bürstenbinder, K.; Semi-Automatic Cell Segmentation from Noisy Image Data for Quantification of Microtubule Organization on Single Cell Level 199-203, (2019) ISBN: 978-1-5386-3641-1 DOI: 10.1109/ISBI.2019.8759145
The structure of the microtubule cytoskeleton provides valuable information related to morphogenesis of cells. The cytoskeleton organizes into diverse patterns that vary in cells of different types and tissues, but also within a single tissue. To assess differences in cytoskeleton organization methods are needed that quantify cytoskeleton patterns within a complete cell and which are suitable for large data sets. A major bottleneck in most approaches, however, is a lack of techniques for automatic extraction of cell contours. Here, we present a semi-automatic pipeline for cell segmentation and quantification of microtubule organization. Automatic methods are applied to extract major parts of the contours and a handy image editor is provided to manually add missing information efficiently. Experimental results prove that our approach yields high-quality contour data with minimal user intervention and serves a suitable basis for subsequent quantitative studies.