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Publikation

Zayneb, C.; Bassem, K.; Zeineb, K.; Grubb, C. D.; Noureddine, D.; Hafedh, M.; Amine, E. Physiological responses of fenugreek seedlings and plants treated with cadmium Environ Sci Pollut Res 22, 10679-10689, (2015) DOI: 10.1007/s11356-015-4270-8

The bioaccumulation efficiency of cadmium (Cd) by fenugreek (Trigonella foenum-graecum) was examined using different concentrations of CdCl2. The germination rate was similar to control except at 10 mM Cd. However, early seedling growth was quite sensitive to the metal from the lowest Cd level. Accordingly, amylase activity was reduced substantially on treatment of seeds with 0.5, 1, and 10 mM Cd. Cadmium also affected various other plant growth parameters. Its accumulation was markedly lower in shoots as compared to roots, reducing root biomass by almost 50 %. Plants treated with 1 and 5 mM Cd presented chlorosis due to a significant reduction in chlorophyll b especially. Furthermore, at Cd concentrations greater than 0.1 mM, plants showed several signs of oxidative stress; an enhancement in root hydrogen peroxide (H2O2) level and in shoot malondialdehyde (MDA) content was observed. Conversely, antioxidant enzyme activities (superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT)) increased in various plant parts. Likewise, total phenolic and flavonoid contents reached their highest values in the 0.5 mM Cd treatment, consistent with their roles in quenching low concentrations of reactive oxygen species (ROS). Consequently, maintaining oxidant and antioxidant balance may permit fenugreek to hyperaccumulate Cd and allow it to be employed in extremely Cd polluted soils for detoxification purposes.
Bücher und Buchkapitel

Wasternack, C. Oxilipins: biosynthesis, signal transduction and action (Hedden, P., Thomas, S.). Ann. Plant Reviews, Blackwell, Oxford, UK 185-228, (2006) DOI: 10.1002/9780470988800.ch7

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Publikation

Sharma, V.K.; Monostori, T.; Hause, B.; Maucher, H.; Göbel, C.; Hornung, E.; Hänsch, R.; Bittner, F.; Wasternack, C.; Feussner, I.; Mendel, R.R.; Schulze, J. Genetic transformation of barley to modify expression of a 13-lipoxygenase Acta Biol. Szeged 49, 33-34 , (2005)

Immature scutella of barley were transformed with cDNA coding for a 13-li-poxygenase of barley (LOX-100) via particle bombardment. Regenerated plants were tested by PAT-assay, Western-analysis and PCR-screening. Immunocytochemical assay of T0 plants showed expression of the LOX cDNA both in the chloroplasts and in the cytosol, depending on the presence of the chloroplast signal peptide sequences in the cDNA. A few transgenic plants containing higher amounts of LOX-derived products have been found. These are the candidates for further analysis concerning pathogen resistance.
Bücher und Buchkapitel

Stenzel, I.; Hause, B.; Feussner, I.; Wasternack, C. Transcriptional activation of jasmonate biosynthesis enzymes is not reflected at protein level (Murata, N., Yamada, M., Nishida, I., Okuyama, H., Sekijar, J., Hajme, W.). Kluwer Academic Publishers 267-270, (2003)

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Bücher und Buchkapitel

Weichert, H.; Maucher, H.; Hornung, E.; Wasternack, C.; Feussner, I. Shift in fatty acid and oxylipin pattern of tomato leaves following overexpression of the allene oxide cyclase (Murata, N., Yamada, M., Nishida, I., Okuyama, H., Sekijar, J., Hajme, W.). Kluwer Academic Publishers 275-278, (2003)

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Publikation

Schilling, S.; Hoffmann, T.; Rosche, F.; Manhart, S.; Wasternack, C.; Demuth, H.-U. Heterologous expression and characterization of human glutaminyl cyclase: evidence for a disulfide bond with importance for catalytic activity Biochemistry 41, 10849-10857, (2002)

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