TY - JOUR ID - 1597 TI - Transcriptional Dynamics of Two Seed Compartments with Opposing Roles in Arabidopsis Seed Germination JO - Plant Physiol PY - 2013 SP - 205-215 AU - Dekkers, B.J.W. AU - Pearce, S. AU - van Bolderen-Veldkamp, R.P. AU - Marshall, A. AU - Widera, P. AU - Gilbert, J. AU - Drost, H.-G. AU - Basseli, G.W. AU - Müller, K. AU - King, J.R. AU - Wood, A.T.A. AU - Grosse, I. AU - Quint, M. AU - Krasnogor, N. AU - Leubner-Metzger, G. AU - Holdsworth, M.J. & Bentsink, L. VL - 163 UR - DO - 10.1104/pp.113.223511 AB - Seed germination is a critical stage in the plant life cycle and the first step toward successful plant establishment. Therefore, understandinggermination is of important ecological and agronomical relevance. Previous research revealed that different seed compartments (testa,endosperm, and embryo) control germination, but little is known about the underlying spatial and temporal transcriptome changes thatlead to seed germination. We analyzed genome-wide expression in germinating Arabidopsis (Arabidopsis thaliana) seedswith both temporaland spatial detail and provide Web-accessible visualizations of the data reported (vseed.nottingham.ac.uk). We show the potential of this highresolutiondata set for the construction ofmeaningful coexpression networks, which provide insight into the genetic control of germination.The data set reveals two transcriptional phases during germination that are separated by testa rupture. The first phase is marked by largetranscriptome changes as the seed switches from a dry, quiescent state to a hydrated and active state. At the end of this first transcriptionalphase, the number of differentially expressed genes between consecutive time points drops. This increases again at testa rupture, the start ofthe second transcriptional phase. Transcriptome data indicate a role for mechano-induced signaling at this stage and subsequently highlightthe fates of the endosperm and radicle: senescence and growth, respectively. Finally, using a phylotranscriptomic approach, we show thatexpression levels of evolutionarily young genes drop during the first transcriptional phase and increase during the second phase.Evolutionarily old genes show an opposite pattern, suggesting a more conserved transcriptome prior to the completion of germination. A2 - C1 - Molecular Signal Processing ER - TY - JOUR ID - 1965 TI - Role of NINJA in root jasmonate signaling JO - PNAS PY - 2013 SP - 15473-15478 AU - Acosta, I.F. AU - Gasperini, D. AU - Chételat, A. AU - Stolz, S. AU - Santuari, L. AU - Farmer, E.E. VL - 110 (38) UR - DO - 10.1073/pnas.1307910110 AB - A2 - C1 - Molecular Signal Processing ER -