Publikationen - Molekulare Signalverarbeitung
Aktive Filter
Autor Nach Häufigkeit alphabetisch sortiert: Monostori, T
Autor Nach Häufigkeit alphabetisch sortiert: Sharma, V.K
Autor Nach Häufigkeit alphabetisch sortiert: Maucher, H
Autor Nach Häufigkeit alphabetisch sortiert: Gray, W.M.
Journal / Buchreihe / Preprint-Server Nach Häufigkeit alphabetisch sortiert: Cell Rep
Autor Nach Häufigkeit alphabetisch sortiert: Ziegler, J.
Autor Nach Häufigkeit alphabetisch sortiert: Quint, M.
Journal / Buchreihe / Preprint-Server Nach Häufigkeit alphabetisch sortiert: Anal. Biochem
Journal / Buchreihe / Preprint-Server Nach Häufigkeit alphabetisch sortiert: Methods Mol. Biol.
Alle Filter entfernen
Suchfilter
- Typ der Publikation
- Erscheinungsjahr
- 2019 (1)
- Journal / Buchreihe / Preprint-Server Nach Häufigkeit alphabetisch sortiert
- Plant J. (9)
- PLOS ONE (8)
- bioRxiv (7)
- Front. Plant Sci. (6)
- Plant Physiol. (5)
- Theor. Appl. Genet. (5)
- BMC Plant Biol. (4)
- 0 (3)
- Curr. Biol. (3)
- J. Biol. Chem. (3)
- Amino Acids (2)
- Int. J. Mol. Sci. (2)
- J. Exp. Bot. (2)
- Mol. Biol. Evol. (2)
- Nat. Plants (2)
- Proc. Natl. Acad. Sci. U.S.A. (2)
- Anal. Biochem. (1)
- BMC Biol. (1)
- BMC Genomics (1)
- Cell (1)
- Cell Rep. (1)
- Curr. Opin. Plant Biol. (1)
- Environ. Exp. Bot. (1)
- Equine Vet. Educ. (1)
- Equine Vet. J. (1)
- Genetika (1)
- J. Chromatogr. A (1)
- J. Plant Growth Regul. (1)
- J. Plant Physiol. (1)
- Journal of Industrial Microbiology and Biotechnology (1)
- Metabolomics (1)
- Methods Mol. Biol. (1)
- Mol. Plant (1)
- Nat. Commun. (1)
- Nature (1)
- Pharmazie (1)
- Phytochemistry (1)
- Plant Cell (1)
- Plant Cell Environ. (1)
- Plant Mol. Biol. (1)
- Plant Pathogens Series (1)
- Planta (1)
- Plants (1)
- Sci. Rep. (1)
- Skin Pharmacol. Physiol. (1)
- Toxins (1)
- Trends Plant Sci. (1)
- Wiley Analytical Science (1)
- eLife (1)
- Autor Nach Häufigkeit alphabetisch sortiert
- Bürstenbinder, K. (2)
- Calderón Villalobos, L. I. A. (2)
- Möller, B. (2)
- Abel, S. (1)
- Gasperini, D. (1)
- Hellmuth, A. (1)
- Hussain, H. (1)
- Klemm, S. (1)
- Mielke, S. (1)
- Neubert, R. H. H. (1)
- Niemeyer, M. (1)
- Parra, J. O. F. (1)
- Poeschl, Y. (1)
- Zergiebel, L. (1)
- Ziegler, J. (1)
Zeige Ergebnisse 1 bis 1 von 1.
Ziegler, J.; Hussain, H.; Neubert, R. H. H.; Abel, S.; Sensitive and Selective Amino Acid Profiling of Minute Tissue Amounts by HPLC/Electrospray Negative Tandem Mass Spectrometry Using 9-Fluorenylmethoxycarbonyl (Fmoc-Cl) Derivatization (Alterman, M. A., ed.). Methods Mol. Biol. 2030, 365-379, (2019) ISBN: 978-1-4939-9639-1 DOI: 10.1007/978-1-4939-9639-1_27
A method for selective and sensitive quantification of amino acids is described. The combination of established derivatization procedures of secondary and primary amino groups with 9-fluorenylmethoxycarbonyl chloride (Fmoc-Cl) and subsequent detection of derivatized amino acids by LC-ESI-MS/MS using multiple reaction monitoring provides high selectivity. The attachment of an apolar moiety enables purification of derivatized amino acids from matrix by a single solid-phase extraction step, which increases sensitivity by reduced ion suppression during LC-ESI-MS/MS detection. Additionally, chromatography of all amino acids can be performed on reversed-phase HPLC columns using eluents without additives, which are known to cause significant decreases in signal to noise ratios. The method has been routinely applied for amino acid profiling of low amounts of liquids and tissues of various origins with a sample throughput of about 50–100 samples a day. In addition to a detailed description of the method, some representative examples are presented.