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Publikationen - Molekulare Signalverarbeitung

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Publikation

Bosch, M.; Wright, L. P.; Gershenzon, J.; Wasternack, C.; Hause, B.; Schaller, A.; Stintzi, A. Jasmonic acid and its precursor 12-oxophytodienoic acid control different aspects of constitutive and induced herbivore defenses in tomato Plant Physiology 166, 396-410, (2014) DOI: 10.1104/pp.114.237388

The jasmonate family of growth regulators includes the isoleucine conjugate of jasmonic acid (JA-Ile) and its biosynthetic precursor 12-oxophytodienoic acid (OPDA) as signaling molecules. In order to assess the relative contribution of JA/JA-Ile and OPDA to insect resistance in tomato, we silenced the expression of OPDA reductase (OPR3) by RNA interference. Consistent with a block in the biosynthetic pathway downstream of OPDA, OPR3-RNAi plants contained wild-type levels of OPDA but failed to accumulate JA or JA-Ile after wounding. JA/JA-Ile deficiency in OPR3-RNAi plants resulted in reduced trichome formation and impaired monoterpene and sesquiterpene production. The loss of these JA/JA-Ile-dependent defense traits rendered them more attractive to the specialist herbivore Manduca sexta with respect to feeding and oviposition. Oviposition preference resulted from reduced levels of repellant mono- and sesquiterpenes. Feeding preference, on the other hand, was caused by increased production of cis-3-hexenal acting as a feeding stimulant for M. sexta larvae in OPR3-RNAi plants. Despite impaired constitutive defenses and increased palatability of OPR3-RNAi leaves, larval development was indistinguishable on OPR3-RNAi and wild-type plants, and much delayed as compared to development on the JA/JA-Ile insensitive (jai1) mutant. Apparently, signaling through JAI1, the tomato ortholog of COI1 in Arabidopsis, is required for defense while the conversion of OPDA to JA/JA-Ile is not. Comparing the signaling activities of OPDA and JA/JA-Ile, we found that OPDA can substitute for JA/JA-Ile in the local induction of defense gene expression, but the production of JA/JA-Ile is required for a systemic response.
Publikation

Guranowski, A.; Miersch, O.; Staswick, P.E.; Suza, W.; Wasternack, C. Substrate specificity and products of side-reactions catalyzed by jasmonate:amino acid synthetase (JAR1) FEBS Letters 581, 815-820, (2007) DOI: 10.1016/j.febslet.2007.01.049

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Publikation

Wasternack, C. Jasmonates - Introductory remarks on biosynthesis and diversity in action J. Plant Growth Reg. 23, 167-169, (2004)

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Publikation

Feussner, I.; Wasternack, C. The lipoxygenase pathway Annu. Rev. Plant Biol. 53, 275-297, (2002)

Lipid peroxidation is common to all biological systems, both appearing in developmentally and environmentally regulated processes of plants. The hydroperoxy polyunsaturated fatty acids, synthesized by the action of various highly specialized forms of lipoxygenases, are substrates of at least seven different enzyme families. Signaling compounds such as jasmonates, antimicrobial and antifungal compounds such as leaf aldehydes or divinyl ethers, and a plant-specific blend of volatiles including leaf alcohols are among the numerous products. Cloning of many lipoxygenases and other key enzymes within the lipoxygenase pathway, as well as analyses by reverse genetic and metabolic profiling, revealed new reactions and the first hints of enzyme mechanisms, multiple functions, and regulation. These aspects are reviewed with respect to activation of this pathway as an initial step in the interaction of plants with pathogens, insects, or abiotic stress and at distinct stages of development.
Publikation

Bohlmann, H.; Vignutelli, A.; Hilpert, B.; Miersch, O.; Wasternack, C.; Apel, K. Wounding and chemicals induce expression of the Arabidopsis gene Thi2.1, encoding a fungal defense thionin, via the octadecanoid pathway FEBS Letters 437, 281-286, (1998)

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Publikation

Görschen, E.; Dunaeva, M.; Reeh, I.; Wasternack, C. Overexpression of the jasmonate inducible 23 kDa protein (JIP 23) from barley in transgenic tobacco leads to the repression of leaf proteins FEBS Letters 419, 58-62, (1997)

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Publikation

Feussner, K.; Feussner, I.; Leopold, I.; Wasternack, C. Isolation of a cDNA coding for an ubiquitin-conjugating enzyme UBCI of tomato - the first stress-induced UBC of higher plants FEBS Letters 409, 211-215, (1997)

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Publikation

Feussner, I.; Kühn, H.; Wasternack, C. Hypothesis. Do specific linoleate 13-lipoxygenases initiate b-oxidation? FEBS Letters 406, 1-5, (1997)

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