Publikationen - Molekulare Signalverarbeitung
Aktive Filter
Autor Nach Häufigkeit alphabetisch sortiert: Monostori, T
Autor Nach Häufigkeit alphabetisch sortiert: Wasternack, C
Autor Nach Häufigkeit alphabetisch sortiert: Maucher, H
Autor Nach Häufigkeit alphabetisch sortiert: Wasternack, C.
Journal / Buchreihe / Preprint-Server Nach Häufigkeit alphabetisch sortiert: Biologie in unserer Zeit
Journal / Buchreihe / Preprint-Server Nach Häufigkeit alphabetisch sortiert: Biol. Chem
Journal / Buchreihe / Preprint-Server Nach Häufigkeit alphabetisch sortiert: Trends Plant Sci.
Autor Nach Häufigkeit alphabetisch sortiert: Feussner, I.
Journal / Buchreihe / Preprint-Server Nach Häufigkeit alphabetisch sortiert: Nat Chem Biol
Erscheinungsjahr: 2001
Erscheinungsjahr: 2009
Journal / Buchreihe / Preprint-Server Nach Häufigkeit alphabetisch sortiert: Nat. Chem. Biol.
Alle Filter entfernen
Suchfilter
- Typ der Publikation
- Publikation (2)
- Erscheinungsjahr
- Journal / Buchreihe / Preprint-Server Nach Häufigkeit alphabetisch sortiert
- Plant Cell (2)
- 0 (1)
- BBA-Mol. Cell Biol. Lipids (1)
- Biochem. Soc. Trans. (1)
- J. Exp. Bot. (1)
- Nat. Chem. Biol. (1)
- New Phytol. (1)
- Phytochemistry (1)
- Plant Cell Physiol. (1)
- Plant Physiol. (1)
- Trends Plant Sci. (1)
- Autor Nach Häufigkeit alphabetisch sortiert
- Wasternack, C. (2)
- Adie, B. (1)
- Calderon-Villalobos, L. I. A. (1)
- Chini, A. (1)
- Estelle, M. (1)
- Feussner, I. (1)
- Fonseca, S. (1)
- Hamberg, M. (1)
- Kramell, R. (1)
- Kühn, H. (1)
- Miersch, O. (1)
- Porzel, A. (1)
- Santner, A. (1)
- Solano, R. (1)
Zeige Ergebnisse 1 bis 2 von 2.
Fonseca, S.; Chini, A.; Hamberg, M.; Adie, B.; Porzel, A.; Kramell, R.; Miersch, O.; Wasternack, C.; Solano, R.; (+)-7-iso-Jasmonoyl-L-isoleucine is the endogenous bioactive jasmonate Nat. Chem. Biol. 5, 344-350, (2009) DOI: 10.1038/nchembio.161
Hormone-triggered activation of the jasmonate signaling pathway in Arabidopsis thaliana requires SCFCOI1-mediated proteasome degradation of JAZ repressors. (−)-JA-L-Ile is the proposed bioactive hormone, and SCFCOI1 is its likely receptor. We found that the biological activity of (−)-JA-L-Ile is unexpectedly low compared to coronatine and the synthetic isomer (+)-JA-L-Ile, which suggests that the stereochemical orientation of the cyclopentanone-ring side chains greatly affects receptor binding. Detailed GC-MS and HPLC analyses showed that the (−)-JA-L-Ile preparations currently used in ligand binding studies contain small amounts of the C7 epimer (+)-7-iso-JA-L-Ile. Purification of each of these molecules demonstrated that pure (−)-JA-L-Ile is inactive and that the active hormone is (+)-7-iso-JA-L-Ile, which is also structurally more similar to coronatine. In addition, we show that pH changes promote conversion of (+)-7-iso-JA-L-Ile to the inactive (−)-JA-L-Ile form, thus providing a simple mechanism that can regulate hormone activity through epimerization.
Feussner, I.; Kühn, H.; Wasternack, C.; Lipoxygenase-dependent degradation of storage lipids Trends Plant Sci. 6, 268-273, (2001) DOI: 10.1016/S1360-1385(01)01950-1
Oilseed germination is characterized by the mobilization of storage lipids as a carbon source for the germinating seedling. In spite of the importance of lipid mobilization, its mechanism is only partially understood. Recent data suggest that a novel degradation mechanism is initiated by a 13-lipoxygenase during germination, using esterified fatty acids specifically as substrates. This 13-lipoxygenase reaction leads to a transient accumulation of ester lipid hydroperoxides in the storage lipids, and the corresponding oxygenated fatty acid moieties are preferentially removed by specific lipases. The free hydroperoxy fatty acids are subsequently reduced to their hydroxy derivatives, which might in turn undergo β-oxidation.