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Publikationen - Molekulare Signalverarbeitung

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Publikation

Fonseca, S.; Chini, A.; Hamberg, M.; Adie, B.; Porzel, A.; Kramell, R.; Miersch, O.; Wasternack, C.; Solano, R. (+)-7-iso-Jasmonoyl-L-isoleucine is the endogenous bioactive jasmonate Nat Chem Biol 5, 344-350, (2009) DOI: 10.1038/nchembio.161

Hormone-triggered activation of the jasmonate signaling pathway in Arabidopsis thaliana requires SCFCOI1-mediated proteasome degradation of JAZ repressors. (-)-JA-L-Ile is the proposed bioactive hormone, and SCFCOI1 is its likely receptor. We found that the biological activity of (-)-JA-L-Ile is unexpectedly low compared to coronatine and the synthetic isomer (+)-JA-L-Ile, which suggests that the stereochemical orientation of the cyclopentanone-ring side chains greatly affects receptor binding. Detailed GC-MS and HPLC analyses showed that the (-)-JA-L-Ile preparations currently used in ligand binding studies contain small amounts of the C7 epimer (+)-7-iso-JA-L-Ile. Purification of each of these molecules demonstrated that pure (-)-JA-L-Ile is inactive and that the active hormone is (+)-7-iso-JA-L-Ile, which is also structurally more similar to coronatine. In addition, we show that pH changes promote conversion of (+)-7-iso-JA-L-Ile to the inactive (-)-JA-L-Ile form, thus providing a simple mechanism that can regulate hormone activity through epimerization.
Publikation

Ederli, L.; Morettini, R.; Borgogni, A.; Wasternack, C.; Miersch, O.; Reale, L.; Ferranti, F.; Tosit, N.; Pasqualini, S. Interaction between nitric oxide and ethylene in the induction of alternative oxidase in ozone-treated tobacco plants Plant Physiol. 142, 595-608, (2006) DOI: 10.1104/pp.106.085472

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Publikation

Mur, L.A.J.; Kenton, P.; Atzorn, R.; Miersch, O.; Wasternack, C. The outcomes of concentration specific interactions between salicylate and jasmonate signaling include synergy, antagonism and the activation of cell death Plant Physiol. 140, 249-262, (2006) DOI: 10.1104/pp.105.072348

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Publikation

O'Donnell, P.J.; Schmelz, E.; Block, A.; Miersch, O.; Wasternack, C.; Jones, J.B.; Klee, H.J. Multiple hormones cooperatively control a susceptible tomato pathogen defense response Plant Physiol. 133, 1181-1189, (2003)

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Publikation

Kramell, R.; Miersch, O.; Atzorn, R.; Parthier, B.; Wasternack, C. Octadecanoid-derived alteration of gene expression and the 'oxylipin signature' in stressed barley leaves - implications for different signalling pathways Plant Physiol. 123, 177-186, (2000)

Stress-induced gene expression in barley (Hordeum vulgare cv. Salome) leaves has been correlated with temporally changing levels of octadecanoids and jasmonates, quantified by means of gas chromatography/mass spectrometry-single ion monitoring. Application of sorbitol-induced stress led to a low and transient rise of jasmonic acid (JA), its precursor 12-oxophytodienoic acid (OPDA) and the methyl esters JAME and OPDAME, respectively, followed by a large increase in their levels. JA and JAME peaked between 12 and 16 h, about 4 hours before OPDA and OPDAME. However, OPDA accumulated up to a 2.5-fold higher level than the other compounds. Dihomo-jasmonic acid and 9,13-didehydro-12- oxophytoenoic acid were identified as minor components. Kinetic analyses revealed that a transient threshold of jasmonates or octadecanoids is necessary and sufficient to initiate JA responsive gene expression. Although OPDA and OPDAME applied exogenously were metabolized to JA in considerable amounts, both of them can induce gene expression per se as evidenced by those genes which do not respond to endogenously formed JA. Also, coronatine induces JA-responsive genes independently from endogenous JA. As evidenced by application of deuterated JA, endogenous synthesis of JA is not induced by JA treatment. The data are discussed in terms of distinct signalling pathways.
Publikation

Herde, O.; Peña-Cortés, H.; Wasternack, C.; Willmitzer, L.; Fisahn, J. Electric signaling and PIN2 gene expression on different abiotic stimuli depend on a distinct threshold level of endogenous ABA in several ABA-deficient tomato mutants Plant Physiol. 119, 213-218, (1999)

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Publikation

Herde, O.; Atzorn, R.; Fisahn, J.; Wasternack, C.; Willmitzer, L.; Peña-Cortés, H. Localized wounding by heat initiates the accumulation of proteinase inhibitor II in abscisic acid-deficient plants by triggering jasmonic acid biosynthesis Plant Physiol. 112, 853-860, (1996)

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