Publikationen - Molekulare Signalverarbeitung
Aktive Filter
Autor Nach Häufigkeit alphabetisch sortiert: Monostori, T
Autor Nach Häufigkeit alphabetisch sortiert: Wasternack, C
Autor Nach Häufigkeit alphabetisch sortiert: Sharma, V.K
Autor Nach Häufigkeit alphabetisch sortiert: Gray, W.M.
Autor Nach Häufigkeit alphabetisch sortiert: Verbeek, M.
Autor Nach Häufigkeit alphabetisch sortiert: Strehmel, N.
Autor Nach Häufigkeit alphabetisch sortiert: Dinesh, D. C.
Autor Nach Häufigkeit alphabetisch sortiert: Kühn, H.
Autor Nach Häufigkeit alphabetisch sortiert: Dagdas, Y.
Journal / Buchreihe / Preprint-Server Nach Häufigkeit alphabetisch sortiert: Proc Natl Acad Sci USA
Journal / Buchreihe / Preprint-Server Nach Häufigkeit alphabetisch sortiert: Plant Physiol
Journal / Buchreihe / Preprint-Server Nach Häufigkeit alphabetisch sortiert: PLOS ONE
Alle Filter entfernen
Suchfilter
- Typ der Publikation
- Publikation (2)
- Erscheinungsjahr
- Journal / Buchreihe / Preprint-Server Nach Häufigkeit alphabetisch sortiert
- 0 (5)
- J. Biol. Chem. (2)
- J. Gen. Virol. (2)
- PLOS ONE (2)
- Trends Plant Sci. (2)
- BBA-Mol. Cell Biol. Lipids (1)
- BIOspektrum (1)
- Curr. Biol. (1)
- EMBO J. (1)
- Eur. J. Biochem. (1)
- FEBS Lett. (1)
- Int. J. Mol. Sci. (1)
- J. Exp. Bot. (1)
- Plant Cell (1)
- Planta (1)
- Proc. Natl. Acad. Sci. U.S.A. (1)
- Sci. Rep. (1)
- bioRxiv (1)
- eLife (1)
- Autor Nach Häufigkeit alphabetisch sortiert
- Quint, M. (4)
- Ziegler, J. (4)
- Abel, S. (2)
- Bochnia, M. (2)
- Gray, W. M. (2)
- Sander, J. (2)
- Wensch-Dorendorf, M. (2)
- Zeyner, A. (2)
- Arnold, M. D. (1)
- Beator, B. (1)
- Bleiss, W. (1)
- Borriss, R. (1)
- Budiharjo, A. (1)
- Buhtz, A. (1)
- Bürstenbinder, K. (1)
- Cavalleri, J.-M. V. (1)
- Chowdhury, S. P. (1)
- Dagdas, Y. (1)
- Delker, C. (1)
- Dharmasiri, N. (1)
- Dharmasiri, S. (1)
- Dietel, K. (1)
- Dolgova, O. (1)
- Fan, B. (1)
- Floková, K. (1)
- Gantner, J. (1)
- Glatter, M. (1)
- Grosch, R. (1)
- Grosse, I. (1)
- Gruber, C. (1)
- Gruetzner, R. (1)
- Hartmann, A. (1)
- Hause, B. (1)
- Huang, H. (1)
- Ilse, T. (1)
- Janzen, N. (1)
- Jayaweera, T. (1)
- Kretschmer, C. (1)
- Löfke, C. (1)
- Marillonnet, S. (1)
- Miersch, O. (1)
- Navarro-Quezada, A. (1)
- Novák, O. (1)
- Ordon, J. (1)
- Poeschl, Y. (1)
- Recknagel, S. (1)
- Sander, S. (1)
- Schaefer, S. (1)
- Schmid, M. (1)
- Schumann, N. (1)
- Schusser, G. F. (1)
- Siriwardana, C. (1)
- Strehmel, N. (1)
- Strnad, M. (1)
- Stuttmann, J. (1)
- Terhardt, M. (1)
- Trenner, J. (1)
- Uhlig, A. (1)
- Ullrich, K. K. (1)
- Vollstedt, S. (1)
- Wasternack, C. (1)
- Witzel, K. (1)
- Zuraw, A. (1)
Zeige Ergebnisse 1 bis 2 von 2.
Gantner, J.; Ordon, J.; Ilse, T.; Kretschmer, C.; Gruetzner, R.; Löfke, C.; Dagdas, Y.; Bürstenbinder, K.; Marillonnet, S.; Stuttmann, J.; Peripheral infrastructure vectors and an extended set of plant parts for the Modular Cloning system PLOS ONE 13, e0197185, (2018) DOI: 10.1371/journal.pone.0197185
Standardized DNA assembly strategies facilitate the generation of multigene constructs from collections of building blocks in plant synthetic biology. A common syntax for hierarchical DNA assembly following the Golden Gate principle employing Type IIs restriction endonucleases was recently developed, and underlies the Modular Cloning and GoldenBraid systems. In these systems, transcriptional units and/or multigene constructs are assembled from libraries of standardized building blocks, also referred to as phytobricks, in several hierarchical levels and by iterative Golden Gate reactions. Here, a toolkit containing further modules for the novel DNA assembly standards was developed. Intended for use with Modular Cloning, most modules are also compatible with GoldenBraid. Firstly, a collection of approximately 80 additional phytobricks is provided, comprising e.g. modules for inducible expression systems, promoters or epitope tags. Furthermore, DNA modules were developed for connecting Modular Cloning and Gateway cloning, either for toggling between systems or for standardized Gateway destination vector assembly. Finally, first instances of a “peripheral infrastructure” around Modular Cloning are presented: While available toolkits are designed for the assembly of plant transformation constructs, vectors were created to also use coding sequence-containing phytobricks directly in yeast two hybrid interaction or bacterial infection assays. The presented material will further enhance versatility of hierarchical DNA assembly strategies.
Buhtz, A.; Witzel, K.; Strehmel, N.; Ziegler, J.; Abel, S.; Grosch, R.; Perturbations in the Primary Metabolism of Tomato and Arabidopsis thaliana Plants Infected with the Soil-Borne Fungus Verticillium dahliae PLOS ONE 10, e0138242, (2015) DOI: 10.1371/journal.pone.0138242
The hemibiotrophic soil-borne fungus Verticillium dahliae is a major pathogen of a number of economically important crop species. Here, the metabolic response of both tomato and Arabidopsis thaliana to V. dahliae infection was analysed by first using non-targeted GC-MS profiling. The leaf content of both major cell wall components glucuronic acid and xylose was reduced in the presence of the pathogen in tomato but enhanced in A. thaliana. The leaf content of the two tricarboxylic acid cycle intermediates fumaric acid and succinic acid was increased in the leaf of both species, reflecting a likely higher demand for reducing equivalents required for defence responses. A prominent group of affected compounds was amino acids and based on the targeted analysis in the root, it was shown that the level of 12 and four free amino acids was enhanced by the infection in, respectively, tomato and A. thaliana, with leucine and histidine being represented in both host species. The leaf content of six free amino acids was reduced in the leaf tissue of diseased A. thaliana plants, while that of two free amino acids was raised in the tomato plants. This study emphasizes the role of primary plant metabolites in adaptive responses when the fungus has colonized the plant.