Publikationen - Molekulare Signalverarbeitung
Aktive Filter
Suchfilter
- Typ der Publikation
- Publikation (1)
- Publikationen in Druck (1)
- Erscheinungsjahr
- 2019 (2)
- Journal / Verlag
- Mol Plant (1)
- Plant Cell Physiol (1)
- bioRxiv (2)
- Autor Nach Häufigkeit alphabetisch sortiert
- Gasperini, D. (2)
- Zimmer, M. (2)
- Calderón Villalobos, L. I. A. (1)
- Dallery, J.-F. (1)
- Gianniou, D. D. (1)
- Halder, V. (1)
- Hause, B. (1)
- Hellmuth, A. (1)
- Hoehenwarter, W. (1)
- Iacobucci, C. (1)
- Ihling, C. H. (1)
- Kastritis, P. L. (1)
- Le Goff, G. (1)
- Melnyk, C. W. (1)
- Mielke, S. (1)
- Moreno Castillo, E. (1)
- Niemeyer, M. (1)
- Ouazzani, J. (1)
- O’Connell, R. J. (1)
- Pigné, S. (1)
- Samodelov, S. L. (1)
- Schulze, A. (1)
- Sinz, A. (1)
- Stellmach, H. (1)
- Suliman, M. (1)
- Trougakos, I. P. (1)
- Wilde, V. (1)
- Zurbriggen, M. D. (1)
Zeige Ergebnisse 1 bis 2 von 2.
Dallery, J.-F.; Zimmer, M.; Halder, V.; Suliman, M.; Pigné, S.; Le Goff, G.; Gianniou, D. D.; Trougakos, I. P.; Ouazzani, J.; Gasperini, D.; O’Connell, R. J. Inhibition of jasmonate-mediated plant defences by the fungal metabolite higginsianin B bioRxiv (2019) DOI: 10.1101/651562
Infection of Arabidopsis thaliana by the
ascomycete fungus Colletotrichum higginsianum is characterised by an
early symptomless biotrophic phase followed by a destructive
necrotrophic phase. The fungal genome contains 77 secondary
metabolism-related biosynthetic gene clusters (BGCs), and their
expression during the infection process is tightly regulated. Deleting
CclA, a chromatin regulator involved in repression of some BGCs through
H3K4 trimethylation, allowed overproduction of 3 families of terpenoids
and isolation of 12 different molecules. These natural products were
tested in combination with methyl jasmonate (MeJA), an elicitor of
jasmonate responses, for their capacity to alter defence gene induction
in Arabidopsis. Higginsianin B inhibited MeJA-triggered expression of
the defence reporter VSP1p:GUS, suggesting it may block bioactive JA-Ile
synthesis or signalling in planta. Using the JA-Ile sensor Jas9-VENUS,
we found that higginsianin B, but not three other structurally-related
molecules, suppressed JA-Ile signalling by preventing degradation of JAZ
proteins, the repressors of JA responses. Higginsianin B likely blocks
the 26S proteasome-dependent degradation of JAZ proteins because it
inhibited chymotrypsin- and caspase-like protease activities. The
inhibition of target degradation by higginsianin B also extended to
auxin signalling, as higginsianin B treatment reduced IAA-dependent
expression of DR5p:GUS. Overall, our data indicate that specific fungal
secondary metabolites can act similarly to protein effectors to subvert
plant immune and developmental responses.
Schulze, A.; Zimmer, M.; Mielke, S.; Stellmach, H.; Melnyk, C. W.; Hause, B.; Gasperini, D. Shoot-to-root translocation of the jasmonate precursor 12-oxo-phytodienoic acid (OPDA) coordinates plant growth responses following tissue damage bioRxiv (2019) DOI: 10.1101/517193
Multicellular organisms rely upon the movement of signaling molecules across cells, tissues and organs to communicate among distal sites. In plants, herbivorous insects, necrotrophic pathogens and mechanical wounding stimulate the activation of the jasmonate (JA) pathway, which in turn triggers the transcriptional changes necessary to protect plants against those challenges, often at the expense of growth. Although previous evidence indicated that JA species can translocate from damaged into distal sites, the identity of the mobile compound(s), the tissues through which they translocate and the consequences of their relocation remain unknown. Here, we demonstrated that endogenous JA species generated after shoot injury translocate to unharmed roots via the phloem vascular tissue in Arabidopsis thaliana. By wounding wild-type shoots of chimeric plants and by quantifying the relocating compounds from their JA-deficient roots, we uncovered that the JA-Ile precursor 12-oxo-phytodienoic acid (OPDA) is a mobile JA species. Our data also showed that OPDA is a primary mobile compound relocating to roots where, upon conversion to the bioactive hormone, it induces JA-mediated gene expression and root growth inhibition. Collectively, our findings reveal the existence of long-distance transport of endogenous OPDA which serves as a communication molecule to coordinate shoot-to-root responses, and highlight the importance of a controlled distribution of JA species among organs during plant stress acclimation.