Bücher und Buchkapitel
Mielke, S.; Gasperini, D. Plant–Insect Bioassay for Testing Arabidopsis Resistance to the Generalist Herbivore Spodoptera littoralis (Champion, A. & Laplaze, L., eds.). Methods Mol Biol 2085, 69-78, (2020) ISBN: 978-1-0716-0142-6 DOI: 10.1007/978-1-0716-0142-6_5
Jasmonates are essential engineers of plant
defense responses against many pests, including herbivorous insects.
Herbivory induces the production of jasmonic acid (JA) and its bioactive
conjugate jasmonoyl-l-isoleucine (JA-Ile), which then triggers a large
transcriptional reprogramming to promote plant acclimation. The
contribution of the JA pathway, including its components and regulators,
to defense responses against insect herbivory can be evaluated by
conducting bioassays with a wide range of host plants and insect pests.
Here, we describe a detailed and reproducible protocol for testing
feeding behavior of the generalist herbivore Spodoptera littoralis on
the model plant Arabidopsis thaliana and hence infer the contribution of
JA-mediated plant defense responses to a chewing insect.
Mielke, S.; Gasperini, D. Interplay between Plant Cell Walls and Jasmonate Production Plant Cell Physiol 60, 2629-2637, (2019) DOI: 10.1093/pcp/pcz119
Plant cell walls are sophisticated
carbohydrate-rich structures representing the immediate contact surface
with the extracellular environment, often serving as the first barrier
against biotic and abiotic stresses. Notably, a variety of perturbations
in plant cell walls result in upregulated jasmonate (JA) production, a
phytohormone with essential roles in defense and growth responses.
Hence, cell wall-derived signals can initiate intracellular JA-mediated
responses and the elucidation of the underlying signaling pathways could
provide novel insights into cell wall maintenance and remodeling, as
well as advance our understanding on how is JA biosynthesis initiated.
This Mini Review will describe current knowledge about cell wall-derived
damage signals and their effects on JA biosynthesis, as well as provide
Gasperini, D.; Acosta, I. F.; Farmer, E. E. Cotyledon Wounding of Arabidopsis Seedlings Bio Protoc 6, e1712, (2016) DOI: 10.21769/BioProtoc.1712
Damage to plant organs through both biotic and
abiotic injury is very common in nature. Arabidopsis thaliana 5-day-old
(5-do) seedlings represent an excellent system in which to study plant
responses to mechanical wounding, both at the site of the damage and in
distal unharmed tissues. Seedlings of wild type, transgenic or mutant
lines subjected to single or repetitive cotyledon wounding can be used
to quantify morphological alterations (e.g., root length, Gasperini et
al., 2015), analyze the dynamics of reporter genes in vivo (Larrieu et
al., 2015; Gasperini et al., 2015), follow transcriptional changes by
quantitative RT-PCR (Acosta et al., 2013; Gasperini et al., 2015) or
examine additional aspects of the wound response with a plethora of
downstream procedures. Here we illustrate how to rapidly and reliably
wound cotyledons of young seedlings, and show the behavior of two
promoters driving the expression of β-glucuronidase (GUS) in entire
seedlings and in the primary root meristem, following single or
repetitive cotyledon wounding respectively. We describe two procedures
that can be easily adapted to specific experimental needs.