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Publikationen - Molekulare Signalverarbeitung

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Publikation

Feussner, I., Hause, B., Nellen, A., Wasternack, C. & Kindl, H. Lipid-body lipoxygenase is expressed in cotyledons during germination prior to other lipoxygenase forms Planta 198, 288-293, (1996) DOI: 10.1007/BF00206255

Lipid bodies are degraded during germination. Whereas some proteins, e.g. oleosins, are synthesized during the formation of lipid bodies of maturating seeds, a new set of proteins, including a specific form of lipoxygenase (LOX; EC 1.13.11.12), is detectable in lipid bodies during the stage of fat degradation in seed germination. In cotyledons of cucumber (Cucumis sativus L.) seedlings at day 4 of germination, the most conspicuous staining with anti-LOX antibodies was observed in the cytosol. At very early stages of germination, however, the LOX form present in large amounts and synthesized preferentially was the lipid-body LOX. This was demonstrated by immunocytochemical staining of cotyledons from 1-h and 24-h-old seedlings: the immunodecoration of sections of 24-h-old seedlings with anti-LOX antiserum showed label exclusively correlated with lipid bodies of around 3 μm in diameter. In accordance, the profile of LOX protein isolated from lipid bodies during various stages of germination showed a maximum at day 1. By measuring biosynthesis of the protein in vivo we demonstrated that the highest rates of synthesis of lipid-body LOX occurred at day 1 of germination. The early and selective appearance of a LOX form associated with lipid bodies at this stage of development is discussed.

Publikation

Abdala, G., Castro, G., Guinazu, M., Tizio, R. & Miersch, O. Occurrence of jasmonic acid in organs of Solanum tuberosum L. c.v. Spunta and its effect on tuberization Plant Growth Reg. 19, 139-143, (1996)

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Publikation

Herde, O., Atzorn, R., Fisahn, J., Wasternack, C., Willmitzer, L. & Peña-Cortés, H. Localized wounding by heat initiates the accumulation of proteinase inhibitor II in abscisic acid-deficient plants by triggering jasmonic acid biosynthesis Plant Physiol. 112, 853-860, (1996)

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Publikation

O'Donnell, P.J., Calvert, C., Atzorn, R., Wasternack, C., Leyser, H.M.O. & Bowles, D.J. Ethylene as a signal mediating the wound response of tomato plants Science 274, 1914-1917, (1996)

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Publikation

Wong, L.M., Abel, S., Shen, N., de la Foata, M., Mal, Y. & Theologis, A. Differential activation of the primary auxin response genes, PS-IAA4/5 and PS-IAA6, during early plant development. Plant Journal 9, 587-599, (1996)

The plant growth hormone auxin typified by indoleacetic acid (IAA) transcriptionally activates early genes in pea, PS-IAA4/5 and PS-IAA6, that are members of a multigene family encoding short-lived nuclear proteins. To gain first insight into the biological role of PS-IAA4/5 and PSIAA6, promoter-β-glucuronidase (GUS) gene fusions were constructed and their expression during early development of transgenic tobacco seedlings was examined. The comparative analysis reveals spatial and temporal expression patterns of both genes that correlate with cells, tissues, and developmental processes known to be affected by auxin. GUS activity in seedlings of both transgenic lines is located in the root meristem, sites of lateral root initiation and in hypocotyls undergoing rapid elongation. In addition, mutually exclusive cell-specific expression is evident. For instance, PS-IAA4/5GUS but not PS-IAA6GUS is expressed in root vascular tissue and in guard cells, whereas only PS-IAA6GUS activity is detectable in glandular trichomes and redistributes to the elongating side of the hypocotyl upon gravitropic stimulation. Expression of PS-IAA4/5 and PS-IAA6 in elongating, dividing, and differentiating cell types indicates multiple functions during development. The common and yet distinct activity patterns of both genes suggest a combinatorial code of spatio-temporal co-expression of the various PS-IAA4/ 5-like gene family members in plant development that may mediate cell-specific responses to auxin.

Publikation

Abel, S., Ballas, N., Wong, L-M. & Theologis, A. DNA elements responsive to auxin Bio Essays 18(8), 647-654, (1996)

Genes induced by the plant hormone auxin are probably involved in the execution of vital cellular functions and developmental processes. Experimental approaches designed to elucidate the molecular mechanisms of auxin action have focused on auxin perception, genetic dissection of the signaling apparatus and specific gene activation. Auxin-responsive promoter elements of early genes provide molecular tools for probing auxin signaling in reverse. Functional analysis of several auxin-specific promoters of unrelated early genes suggests combinatorial utilization of both conserved and variable elements. These elements are arranged into autonomous domains and the combination of such modules generates uniquely composed promoters. Modular promoters allow for auxin-mediated transcriptional responses to be revealed in a tissue- and development-specific manner.

Publikation

Abel, S. & Theologis, A. Early genes and auxin action Plant Physiology 111, 9-17, (1996)

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Publikation

Feussner, K., Guranowski, A., Kostka, S. & Wasternack, C. Diadenosine 5'5'''-P1,P4-tetraphosphate (Ap4A) hydrolase from tomato (Lycopersicon esculentum cv. Lukullus) - Purification, Biochemical properties and behaviour during stress Z. Naturforsch. 51c, 477-486, (1996)

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Publikation

Peña-Cortés, H., Prat, S., Atzorn, R., Wasternack, C. & Willmitzer, L. Pin2 gene expression in potato and tomato detached leaves from ABA-deficient potato and tomato plants upon systemin treatment Planta 198, 447-451, (1996)

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Publikation

Wasternack, C., Atzorn, R., Pena-Cortes, H. & Parthier, B. Alteration of gene expression by jasmonate and ABA in tobacco and tomato J. Plant Physiol. 147, 503-510, (1996)

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