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Imaging Unit

Nowadays, the elucidation of molecular and biochemical processes requires its combination with investigation of cells – their physiological properties, their structure, the organelles they contain, interactions with their environment, their life cycle, division and death. For centuries, progress in biological research has been connected to the development of tools and equipment that allow new insights into living matter. The invention of and improvements in optical systems were very important because exceeding the limits of the optical resolution of the human eye delivered new insights into tissues, cells and subcellular compartments on the one hand and cellular processes on the other.

The Imaging Unit of the IPB aims to support all research groups in their use of cell biological methods. Currently, at least 13 research groups of the institute are using this unit.

The unit provides:

  • Coordinated supervision and maintenance of equipment
  • Optimal training of coworkers
  • Maximal use of IPB investments
  • Updating of equipment according to state-of-the-art and to requirements of actual research

The working principle of this unit is:

  • Unit headed by one scientist (Prof. Dr. Bettina Hause) and supported by one technician (Hagen Stellmach)
  • Equipment is located decentralized, but its maintenance is carried out centralized
  • For all cell biological methods advice, training and help is provided, extensive experiments, however, have to be done by the co-workers themselves

Devices and materials:

 

Microscopes:

Several stereo microscopes
(Zeiss and Nikon)

Multipurpose MacroMicroSystem equipped with epi-fluorescence: AZ100 (Nikon) with camera (one in each Dept. MSV and SEB)

Lightsheet Microscope
 

Lightsheet Z1 (Zeiss)                                                                                                                                               

Epi-fluorescence microscopes

Axioplan 2 (Zeiss) with differential interference contrast (DIC) device and ApoTome to obtain optical sections, with two cameras (AxioCam MRm and AxioCam MRc5)

AxioImager (Zeiss) with differential interference contrast (DIC) device and ApoTome to obtain optical sections, with two cameras (AxioCam MRm and AxioCam MRc5)

Confocal Laser Scanning Microscope

LSM780 (Zeiss) with Airyscan

LSM700 (Zeiss)


Microtomes

Rotary microtomes to perform semi-thin sections (Microm und Leica)

Vibrating microtome (Vibratome VT1000S, Leica) (Dept. SZB)

Cryo-Microtom CM1950 (Leica)                                                                      

Miscellaneous

InsituPro VSi (Intavis) for automated in situ detection (Dept. SZB)

Micromanipulator (Eppendorf)

Laser Capture Microdissection                                                                    

More devices

  • Organelle-marker: Vectors and transgenic lines of Arabidopsis (Nelson et al., 2007)
  • Wave-marker: Vectors and transgenic lines of Arabidopsis (Geldner et al., 2009)









Methods established:

  • Fixiation, embedding and sectioning of plant materials
  • Laser-Micro-Dissection
  • Immuno labelling
  • in situ-hybridisation
  • light microscopy including fluorescence microscopy
  • confocal laser scanning microscopy
  • Determination of protein interactions via FRET and BiFC (Split-YFP)

Publications by Tag: Cell Biology

Displaying results 1 to 1 of 1.

Publications

Fellenberg, C.; van Ohlen, M.; Handrick, V.; Vogt, T. The role of CCoAOMT and COMT in Arabidopsis anthers. Planta 236, 51-61, (2012)

Arabidopsis caffeoyl coenzyme A dependent O-methyltransferase 1 (CCoAOMT1) and caffeic acid O-methyltransferase 1 (COMT1) display a similar substrate profile although with distinct substrate preferences and are considered the key methyltransferases (OMTs) in the biosynthesis of lignin monomers, coniferyl and sinapoylalcohol. Whereas CCoAOMT1 displays a strong preference for caffeoyl coenzyme A, COMT1 preferentially methylates 5-hydroxyferuloyl CoA derivatives and also performs methylation of flavonols with vicinal aromatic dihydroxy groups, such as quercetin. Based on different knockout lines, phenolic profiling, and immunohistochemistry, we present evidence that both enzymes fulfil distinct, yet different tasks in Arabidopsis anthers. CCoAOMT1 besides its role in vascular tissues can be localized to the tapetum of young stamens, contributing to the biosynthesis of spermidine phenylpropanoid conjugates. COMT1, although present in the same organ, is not localized in the tapetum, but in two directly adjacent cells layers, the endothecium and the epidermal layer of stamens. In vivo localization and phenolic profiling of comt1 plants provide evidence that COMT1 neither contributes to the accumulation of spermidine phenylpropanoid conjugates nor to the flavonol glycoside pattern of pollen grains.

This page was last modified on 14.11.2018.

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