TY - CHAP ID - 1 TI - Quantitative analysis of microtubule organization in leaf epidermis pavement cells T2 - Methods in Molecular Biology PB - The Plant Cytoskeleton PY - 2023 SP - 43-61 AU - Klemm, S. AU - Buhl, J. AU - Möller, B. AU - Bürstenbinder, K. AU - VL - 2604 UR - https://doi.org/10.1007/978-1-0716-2867-6_4 SN - 978-1-0716-2866-9 DO - 10.1007/978-1-0716-2867-6_4 AB - Leaf epidermis pavement cells form highly complex shapes with interlocking lobes and necks at their anticlinal walls. The microtubule cytoskeleton plays essential roles in pavement cell morphogenesis, in particular at necks. Vice versa, shape generates stress patterns that regulate microtubule organization. Genetic or pharmacological perturbations that affect pavement cell shape often affect microtubule organization. Pavement cell shape and microtubule organization are therefore closely interconnected. Here, we present commonly used approaches for the quantitative analysis of pavement cell shape characteristics and of microtubule organization. In combination with ablation experiments, these methods can be applied to investigate how different genotypes (or treatments) affect the organization and stress responsiveness of the microtubule cytoskeleton. A2 - Hussey, P.J., Wang, P. C1 - Molecular Signal Processing ER -