@Article{IPB-400,
author = {Vendemiatti, E. and Therezan, R. and Vicente, M. and Pinto, M. and Bergau, N. and Yang, L. and Bernardi, W. and Alencar, S. and Zsögön, A. and Tissier, A. and Benedito, V. and Peres, L.},
title = {{The genetic complexity of type-IV trichome development reveals the steps towards an insect-resistant tomato}},
year = {2022},
pages = {1309},
journal = {Plants},
doi = {10.3390/plants11101309},
url = {https://doi.org/10.3390/plants11101309},
volume = {11},
abstract = {The leaves of the wild tomato Solanum galapagense harbor type-IV glandular trichomes (GT) that produce high levels of acylsugars (AS), conferring insect resistance. Conversely, domesticated tomatoes (S. lycopersicum) lack type-IV trichomes on the leaves of mature plants, preventing high AS production, thus rendering the plants more vulnerable to insect predation. We hypothesized that cultivated tomatoes engineered to harbor type-IV trichomes on the leaves of adult plants could be insect-resistant. We introgressed the genetic determinants controlling type-IV trichome development from S. galapagense into cv. Micro-Tom (MT) and created a line named “Galapagos-enhanced trichomes” (MT-Get). Mapping-by-sequencing revealed that five chromosomal regions of S. galapagense were present in MT-Get. Further genetic mapping showed that S. galapagense alleles in chromosomes 1, 2, and 3 were sufficient for the presence of type-IV trichomes on adult organs but at lower densities. Metabolic and gene expression analyses demonstrated that type-IV trichome density was not accompanied by the AS production and exudation in MT-Get. Although the plants produce a significant amount of acylsugars, those are still not enough to make them resistant to whiteflies. We demonstrate that type-IV glandular trichome development is insufficient for high AS accumulation. The results from our study provided additional insights into the steps necessary for breeding an insect-resistant tomato.}    
}