Books and chapters
Mielke, S.; Gasperini, D. Plant–Insect Bioassay for Testing Arabidopsis Resistance to the Generalist Herbivore Spodoptera littoralis (Champion, A. & Laplaze, L., eds.). Methods Mol Biol 2085, 69-78, (2020) ISBN: 978-1-0716-0142-6 DOI: 10.1007/978-1-0716-0142-6_5
Jasmonates are essential engineers of plant
defense responses against many pests, including herbivorous insects.
Herbivory induces the production of jasmonic acid (JA) and its bioactive
conjugate jasmonoyl-l-isoleucine (JA-Ile), which then triggers a large
transcriptional reprogramming to promote plant acclimation. The
contribution of the JA pathway, including its components and regulators,
to defense responses against insect herbivory can be evaluated by
conducting bioassays with a wide range of host plants and insect pests.
Here, we describe a detailed and reproducible protocol for testing
feeding behavior of the generalist herbivore Spodoptera littoralis on
the model plant Arabidopsis thaliana and hence infer the contribution of
JA-mediated plant defense responses to a chewing insect.