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Publications - Molecular Signal Processing

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Displaying results 1 to 9 of 9.

Publications

Flores, R.; Delgado, S.; Gas, M.E.; Carbonell, A.; Molina, D.; Gago, S.; de la Peña, M. Viroids: the minimal non-coding RNA's with autonomous replication FEBS Letters 567, 42-48, (2004) DOI: 10.1016/j.febslet.2004.03.118

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Publications

Carbonell, A.; de la Peña, M.; Flores, R.; Gago, S. Effects of the trinucleotide preceding the self-cleavage site on eggplant latent viroid hammerheads: difference in co- and post-transcriptional self-cleavage may explain the lack trinucleotide AUC in most natural hammerheads Nucleic Acids Research 34, 5613-5622, (2006) DOI: 10.1093/nar/gkl717

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Books and chapters

Flores, R.; Carbonell, A.; de la Peña, M.; Gago, S. RNAs autocatalíticos: ribozimas de cabeza de martillo (Fenoll, C., Marcos, J., Pallás, V., Rodriguez Palenzuela, P.). Spanish Society of Phytopathology 407-420, (2007) ISBN: 84-6476-319-6

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Books and chapters

Flores, R.; Carbonell, A.; Gago, S.; Martínez de Alba, A.E.; Delgado, S.; Rodio, M.E.; di Serio, F. Viroid-host interactions: A molecular dialogue between two uneven partners (Lorito, M., Woo, S. L., Scala, F.). 6 (chap. 58), 1-9, (2008)

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Publications

Carbonell, A.; Martínez de Alba, A.E.; Flores, R.; Gago, S. Double-stranded RNA interferes in a sequence-specific manner with the infection of representative members of the two viroid families Virology 371, 44-53, (2008) DOI: 10.1016/j.virol.2007.09.031

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Publications

Flores, R.; Gas, M.E.; Molina-Serrano, D.; Nohales, M.A.; Carbonell, A.; Gago, S.; de la Peña, M.; Daròs, J.A. Viroid replication: rolling-circles, enzymes and ribozymes Viruses 1, 317-334, (2009) DOI: 10.3390/v1020317

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Publications

Carbonell, A.; Flores, R.; Gago, S. Trans -cleaving hammerhead ribozymes with tertiary stabilizing motifs: in vitro and in vivo activity against a structured viroid RNA Nucleic Acids Research 39, 2432-2444, (2011) DOI: 10.1093/nar/gkq1051

Trans -cleaving hammerheads with discontinuous or extended stem I and with tertiary stabilizing motifs (TSMs) have been tested previously against short RNA substrates in vitro at low Mg 2+ concentration. However, the potential of these ribozymes for targeting longer and structured RNAs in vitro and in vivo has not been examined. Here, we report the in vitro cleavage of short RNAs and of a 464-nt highly structured RNA from potato spindle tuber viroid (PSTVd) by hammerheads with discontinuous and extended formats at submillimolar Mg 2+ . Under these conditions, hammerheads derived from eggplant latent viroid and peach latent mosaic viroid (PLMVd) with discontinuous and extended formats, respectively, where the most active. Furthermore, a PLMVd-derived hammerhead with natural TSMs showed activity in vivo against the same long substrate and interfered with systemic PSTVd infection, thus reinforcing the idea that this class of ribozymes has potential to control pathogenic RNA replicons.
Books and chapters

Carbonell, A.; Flores, R.; Gago, S. Hammerhead Ribozymes Against Virus and Viroid RNAs (Erdmann, V. A. & Barciszewski, J., eds.). RNA Technologies 411-427, (2012) ISBN: 978-3-642-27426-8 DOI: 10.1007/978-3-642-27426-8_16

The hammerhead ribozyme, a small catalytic motif that promotes self-cleavage of the RNAs in which it is found naturally embedded, can be manipulated to recognize and cleave specifically in trans other RNAs in the presence of Mg2+. To be really effective, hammerheads need to operate at the low concentration of Mg2+ existing in vivo. Evidence has been gathered along the last years showing that tertiary stabilizing motifs (TSMs), particularly interactions between peripheral loops, are critical for the catalytic activity of hammerheads at physiological levels of Mg2+. These TSMs, in two alternative formats, have been incorporated into a new generation of more efficient trans-cleaving hammerheads, some of which are active in vitro and in planta when targeted against the highly structured RNA of a viroid (a small plant pathogen). This strategy has potential to confer protection against other RNA replicons, like RNA viruses infecting plants and animals.
Publications

Serra, P.; Carbonell, A.; Navarro, B.; Gago-Zachert, S.; Li, S.; Di Serio, F.; Flores, R. Symptomatic plant viroid infections in phytopathogenic fungi: A request for a critical reassessment Proc Natl Acad Sci USA 117, 10126-10128, (2020) DOI: 10.1073/pnas.1922249117

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