Publications - Molecular Signal Processing
Search narrowed by
Author Sorted by frequency and by alphabetical order: Monostori, T
Author Sorted by frequency and by alphabetical order: Wasternack, C
Author Sorted by frequency and by alphabetical order: Sharma, V.K
Author Sorted by frequency and by alphabetical order: Maucher, H
Author Sorted by frequency and by alphabetical order: Wasternack, C.
Author Sorted by frequency and by alphabetical order: Porzel, A.
Journal / Volume / Preprint Server Sorted by frequency and by alphabetical order: Phytochemistry
Journal / Volume / Preprint Server Sorted by frequency and by alphabetical order: New Biotechnology
Journal / Volume / Preprint Server Sorted by frequency and by alphabetical order: Curr Opin Plant Biol.
Author Sorted by frequency and by alphabetical order: Feussner, I.
Journal / Volume / Preprint Server Sorted by frequency and by alphabetical order: J Exp Bot
Journal / Volume / Preprint Server Sorted by frequency and by alphabetical order: Phytochemistry
Remove all filters
Advanced Search
- Type of publication
- Publication (2)
- Year
- Journal / Volume / Preprint Server Sorted by frequency and by alphabetical order
- Phytochemistry (2)
- FEBS Lett. (1)
- Author Sorted by frequency and by alphabetical order
- Wasternack, C. (2)
- Fester, T. (1)
- Floková, K. (1)
- Groß, N. (1)
- Halfmann, K. (1)
- Köck, M. (1)
- Lohse, S. (1)
- Miersch, O. (1)
- Novák, O. (1)
- Strnad, M. (1)
- Tarkowská, D. (1)
Displaying results 1 to 2 of 2.
Floková, K.; Tarkowská, D.; Miersch, O.; Strnad, M.; Wasternack, C.; Novák, O.; UHPLC–MS/MS based target profiling of stress-induced phytohormones Phytochemistry 105, 147-157, (2014) DOI: 10.1016/j.phytochem.2014.05.015
Stress-induced changes in phytohormone metabolite profiles have rapid effects on plant metabolic activity and growth. The jasmonates (JAs) are a group of fatty acid-derived stress response regulators with roles in numerous developmental processes. To elucidate their dual regulatory effects, which overlap with those of other important defence-signalling plant hormones such as salicylic acid (SA), abscisic acid (ABA) and indole-3-acetic acid (IAA), we have developed a highly efficient single-step clean-up procedure for their enrichment from complex plant matrices that enables their sensitive quantitative analysis using hyphenated mass spectrometry technique. The rapid extraction of minute quantities of plant material (less than 20 mg fresh weight, FW) into cold 10% methanol followed by one-step reversed-phase polymer-based solid phase extraction significantly reduced matrix effects and increased the recovery of labile JA analytes. This extraction and purification protocol was paired with a highly sensitive and validated ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method and used to simultaneously profile sixteen stress-induced phytohormones in minute plant material samples, including endogenous JA, several of its biosynthetic precursors and derivatives, as well as SA, ABA and IAA.
Groß, N.; Wasternack, C.; Köck, M.; Wound-induced RNaseLE expression is jasmonate and systemin independent and occurs only locally in tomato (Lycopersicon esculentum cv. Lukullus) Phytochemistry 65, 1343-1350, (2004) DOI: 10.1016/j.phytochem.2004.04.036
Tomato RNaseLE is induced by phosphate deficiency and wounding and may play a role in macromolecular recycling as well as wound healing. Here, we analyzed the role of jasmonate and systemin in the wound-induced RNaseLE activation. The rapid expression of RNaseLE upon wounding of leaves leading to maximal RNase activity within 10 h, appeared only locally. Jasmonic acid (JA) or its molecular mimic ethyl indanoyl isoleucine conjugate did not induce RNaseLE expression. Correspondingly, RNaseLE was expressed upon wounding of 35S::allene oxide cyclase antisense plants known to be JA deficient. RNaseLE was not expressed upon systemin treatment, but was locally expressed in the spr1 mutant which is affected in systemin perception. In tomato plants carrying a PromLE::uidA construct, GUS activity could be detected upon wounding, but not following treatment with JA or systemin. The data indicate a locally acting wound-inducible systemin- and JA-independent signaling pathway for RNaseLE expression.RNaseLE expression was analyzed by pharmacological studies of different tomato lines and upon wounding of leaves. The gene is only locally activated via a new type of wound-induced signaling pathway in a jasmonate/systemin-independent manner.