Jasmonate Function & Mycorrhiza

Jasmonates are synthesized from α-linolenic acid released from plastid membranes. The first three steps of the JA biosynthesis leading to the intermediate cis-12-oxo-phytodienoic acid (OPDA) are located in plastids. The conversion of OPDA to JA, however, is located in peroxisomes. Since OPDA itself is a molecule with signaling potential, it is likely that its movement between subcellular compartments is highly regulated. The aim of this project is to answer the question, whether JA biosynthesis is dependent on plastid development and peroxisome proliferation. Transgenic plants expressing fluorescent proteins localized to both organelles will be analyzed by confocal laser scanning microscopy to visualize the dynamics of both types of organelles upon stress treatment leading to synthesis of JA (e.g., wounding). In addition, mutants affected in organelle movement and/or morphology as well as wild type plant tissues treated with chemicals affecting peroxisome proliferation will be used to monitor their capacity to synthesize OPDA and JA. This project is funded in frame of the RTG 2498 by the DFG.