+++ News Ticker Science #65 +++ Proteomics +++
Seed Proteomics Methods: Challenges and Perspectives.
IPB experts in collaboration with Russian colleagues have recently published a review on methods in seed proteomics. Seeds of cereals and legumes rich in oils, proteins, and carbohydrates dominate modern agriculture and play an essential role in food industry and fuel production. The seed proteome represents a highly-complex system, only a part of which has been sufficiently described to date. With a broad panel of analytical, biochemical, and physiological methods, scientists are trying to better understand seed development, vigor, longevity, nutritional value, and safety. Proteomics represents one of the most effective methodological platforms giving access to cellular metabolism at the level of proteins.
The authors provide an overview on seed proteomics with a special emphasis on sample preparation, data acquisition, processing, and post-processing. They state, that the depth of seed proteome characterization needs to be increased for a better understanding of the processes accompanying seed development and germination. To improve the proteome coverage and to allow cross-validation of the obtained data, they suggest to simultaneously consider the datasets acquired by LC-MS and 2D-GE which can lead to overall improved result reliability. In their review, the authors not only discuss methods already employed in seed proteomics, but also those still waiting for implementation in this field of plant research. Thereby, they aim to direct the attention of seed biologists to new methodologies emerging in different areas of proteomics research, e.g., clinical or ecological proteomics.
Smolikova, G.; Gorbach, D.; Lukasheva, E.; Mavropolo-Stolyarenko, G.; Bilova, T.; Soboleva, A.; Tsarev, A.; Romanovskaya, E.; Podolskaya, E.; Zhukov, V.; Tikhonovich, I.; Medvedev, S.; Hoehenwarter, W.; Frolov, A. Bringing New Methods to the Seed Proteomics Platform: Challenges and Perspectives. Int. J. Mol. Sci. 2020, 21, 9162. https://doi.org/10.3390/ijms21239162