+++ News Ticker Science #15 +++ Plant-Insect Bioassay +++
Plant-Insect Bioassay for Testing Arabidopsis Resistance to the Herbivore S. littoralis.
Jasmonates are essential engineers of plant defense responses against many pests, including herbivorous insects. Herbivory induces the production of jasmonic acid (JA) and its bioactive conjugate jasmonoyl-L-isoleucine (JA-Ile), which then triggers a large transcriptional reprogramming to promote plant acclimation. The contribution of the JA pathway, including its components and regulators, to defense responses against insect herbivory can be evaluated by conducting bioassays with a wide range of host plants and insect pests. IPB scientists describe a detailed and reproducible protocol for testing feeding behavior of the generalist herbivore Spodoptera littoralis on the model plant Arabidopsis thaliana and hence infer the contribution of JA-mediated plant defense responses to a chewing insect.
Mielke, S. & Gasperini, D. Plant-Insect Bioassay for Testing Arabidopsis Resistance to the Generalist Herbivore Spodoptera littoralis. Methods in Molecular Biology: Jasmonate in Plant Biology, pp. 69-78.