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Technische Ausstattung

Metabolomics

LC-MS/MS

UPLC (Acquity, Waters) - QTrap 6500 (Sciex)        

  • targeted LC-MS/MS of
    - central carbon metabolites
    - phytohormones
    - carotenoids

LDTD Ion Source

Laser Diode Thermal Desorption Ion Source (Phytronix)

  • direct sample introduction source requiring no chromatographic separations prior to detection via MS
  • e.g., for high-throughput screening of clone libraries

LC-MS

UPLC with absorbance, fluoresence and MS detection (Waters)

GC-MS

GCMS-QP2010 Ultra (Shimadzu)

  • quadrupole-based GC-MS of volatile terpenoids
  • thermo-desorption (TD-20, Shimadzu)

LC-MS/MS

UPLC (Acquity, Waters) - TripleToF 5600 (Sciex)

  • untargeted LC-HR-SWATH-MS/MS of
    - plant secondary metabolites
    - hydrophilic/charged metabolites
     

Ion Chromatography System

Ion Chromatograph ICS-5000+ (Dionex, Thermo)

  • cation exchange chromatography
  • anion exchange chromatography
    both coupled to
    1. post-column ion suppression and
    2. mass spectrometry

Online SPE-UPLC

Online solid phase extraction - UPLC (Symbiosis Pico, Spark Holland)

  • sample clean-up
  • ultra-sensitive phytohormone analysis

GC-MS

GC (TraceGC Ultra, Thermo) - MS (SQ, Thermo) with Atlas Injector

Proteinexpression und -aufreinigung

Fermenter

Bench-top bioreactor (Labfors 5, Infors HT) with Cleaning and sterilisation unit (LabCIP, Infors HT)

High pressure cell disruption

French Press TS 0.75 (Constant Systems)

FPLC

ÄKTA (GE Healthcare)

Expressionsanalysen

RNA Qualitätskontrolle

QIAxcel

MicroArray

Affymetrix GeneAtlas Personal MicroArray System

Real-Time PCR

BioRad Realtime System

Weitere Geräte

Mikroplattenreader TECAN

Mikrovolumen Spektrophotometer

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