@Article{IPB-2088, author = {Krajinski, F. and Hause, B. and Gianinazzi-Pearson, V. and Franken, P.}, title = {{Mtha1, a Plasma Membrane H\+-ATPase Gene from Medicago truncatula, Shows Arbuscule-Specific Induced Expression in Mycorrhizal Tissue}}, year = {2003}, pages = {754-761}, journal = {Plant Biol.}, doi = {10.1055/s-2002-37407}, volume = {4}, abstract = {Transport processes between plant and fungal cells are key elements in arbuscular mycorrhiza (AM), where H\+‐ATPases are considered to be involved in active uptake of nutrients from the symbiotic interface. Genes encoding H\+‐ATPases were identified in the genome of Medicago truncatula and three cDNA fragments of the H\+‐ATPase gene family (Mtha 1 ‐ 3) were obtained by RT‐PCR using RNA from M. truncatula mycorrhizal roots as template. While Mtha 2 and Mtha 3 appeared to be constitutively expressed in roots and unaffected by AM development, transcripts of Mtha 1 could only be detected in AM tissues and not in controls. Further analyses by RT‐PCR revealed that Mtha 1 transcripts are not detectable in shoots and phosphate availability did not affect RNA accumulation of the gene. Localization of transcripts by in situ hybridization on AM tissues showed that Mtha 1 RNA accumulates only in cells containing fungal arbuscules. This is the first report of arbuscule‐specific induced expression of a plant H\+‐ATPase gene in mycorrhizal tissues.} }