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Publikationen - Molekulare Signalverarbeitung

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Bücher und Buchkapitel

Flores, R., Carbonell, A., de la Peña, M. & Gago, S. RNAs autocatalíticos: ribozimas de cabeza de martillo. In: Herramientas Biotecnológicas en Fitopatología (Fenoll, C., Marcos, J., Pallás, V., Rodriguez Palenzuela, P.). Spanish Society of Phytopathology 407-420, (2007) ISBN: 84-6476-319-6

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Publikation

Calderón Villalobos, L.I., Nill, C., Marrocco, K., Kretsch, T. & Schwechheimer, C. The evolutionarily conserved Arabidopsis thaliana F-box protein AtFBP7 is required for efficient translation during temperature stress Gene 392(1-2), 106-116, (2007)

In eukaryotes, E3 ubiquitin ligases (E3s) mediate the ubiquitylation of proteins that are destined for degradation by the ubiquitin-proteasome system. In SKP1/CDC53/F-box protein (SCF)-type E3 complexes, the interchangeable F-box protein confers specificity to the E3 ligase through direct physical interactions with the degradation substrate. The vast majority of the approximately 700 F-box proteins from the plant model organism Arabidopsis thaliana remain to be characterized. Here, we investigate the previously uncharacterized and evolutionarily conserved Arabidopsis F-box protein 7 (AtFBP7), which is encoded by a unique gene in Arabidopsis (At1g21760). Several apparent fbp7 loss-of-function alleles do not have an obvious phenotype. AtFBP7 is ubiquitously expressed and its expression is induced after cold and heat stress. When following up on a reported co-purification of the eukaryotic elongation factor-2 (eEF-2) with YLR097c, the apparent budding yeast orthologue of AtFBP7, we discovered a general defect in protein biosynthesis after cold and heat stress in fbp7 mutants. Thus, our findings suggest that AtFBP7 is required for protein synthesis during temperature stress.

Publikation

Lannoo, N., Vandenborre, G., Miersch, O., Smagghe, G., Wasternack, C., Peumans, W.J. & Van Damme, E.J.M. The jasmonate-induced expression of the Nicotiana tabacum leaf lectin Plant and Cell Physiol. 48, 1207-1218, (2007)

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Publikation

Abel, S. Auxin is surfacing ACS Chemical Biology 2, 380 - 384, (2007)

Indole-3-acetic acid (IAA or auxin) is essential throughout the life cycle of a plant. It controls diverse cellular processes, including gene expression. The hormone is perceived by a ubiquitin protein ligase (E3) and triggers the rapid destruction of repressors, called Aux/IAA proteins. The first structural model of a plant hormone receptor illustrates how auxin promotes Aux/IAA substrate recruitment by extending the hydrophobic protein-interaction surface. This work establishes a novel mechanism of E3 regulation by small molecules and promises a novel strategy for the treatment of human disorders associated with defective ubiquitin-dependent proteolysis.

Publikation

Tan, X., Calderón Villalobos, L.I., Sharon, M., Zheng, C., Robinson, C.V., Estelle, M. & Zheng, N. Mechanism of auxin perception by the TIR1 ubiquitin ligase Nature 446(7136), 640-645, (2007)

Arabidopsis

Auxin is a pivotal plant hormone that controls many aspects of plant growth and development. Perceived by a small family of F-box proteins including transport inhibitor response 1 (TIR1), auxin regulates gene expression by promoting SCF ubiquitin-ligase-catalysed degradation of the Aux/IAA transcription repressors, but how the TIR1 F-box protein senses and becomes activated by auxin remains unclear. Here we present the crystal structures of the

TIR1–ASK1 complex, free and in complexes with three different auxin compounds and an Aux/IAA substrate peptide. These structures show that the leucine-rich repeat domain of TIR1 contains an unexpected inositol hexakisphosphate co-factor and recognizes auxin and the Aux/IAA polypeptide substrate through a single surface pocket. Anchored to the base of the TIR1 pocket, auxin binds to a partially promiscuous site, which can also accommodate various auxin analogues. Docked on top of auxin, the Aux/IAA substrate peptide occupies the rest of the TIR1 pocket and completely encloses the hormone-binding site. By filling in a hydrophobic cavity at the protein interface, auxin enhances the TIR1–substrate interactions by acting as a ‘molecular glue’. Our results establish the first structural model of a plant hormone receptor.

Publikation

Wasternack, C. Jasmonates: An update on biosynthesis, signal transduction and action in plant stress response, growth and development Annals of Botany 100, 681-697, (2007)

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Publikation

Delker, C., Zolman, B.K., Miersch, O. & Wasternack, C. Jasmonate biosynthesis in Arabidopsis thaliana requires peroxisomal beta-oxidation enzymes Additional proof by properties of pex6 and aim1 Phytochemistry 68, 1642-1650, (2007)

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Publikation

Schilling, S., Stenzel, I., von Bohlen, A., Wermann, M., Schulz, K., Demuth, H.-U. & Wasternack, C. Isolation and characterization of the glutaminyl cyclases from Solanum tuberosum and Arabidopsis thaliana: implications for physiological functions Biol. Chem 388, 145-153, (2007)

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Publikation

Vigliocco, A., Alemano, S., Miersch, O., Alvarez, D. & Abdala, G. Endogenous jasmonates in dry and imbibed sunflower seeds from plants grown at different soil moisture contents Seed Sci. Res. 17, 91-98, (2007)

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Publikation

Flores, R., Navarro, B., Gago, S. & de la Peña, M. Chrysanthemum Chlorotic Mottle Viroid: a System for Reverse Genetics in the Family Avsunviroidae (Hammerhead Viroids) Plant Viruses 1, 27-32, (2007)

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