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Publikationen - Molekulare Signalverarbeitung

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Publikation

Abel, S. & Theologis, A. A polymorphic bipartite motif signals nuclear targeting of early auxin- inducible proteins related to PS-IAA4 from pea (Pisum sativum) Plant Journal 8, 87-96, (1995)

The plant hormone, indoleacetic acid (IAA), transcriptionally activates two early genes in pea, PS-IAA4/5 and PS-IAA6, that encode short-lived nuclear proteins. The identification of the nuclear localization signals (NLS) in PS-IAA4 and PS-IAA6 using progressive deletion analysis and site-directed mutagenesis is reported. A C-terminal SV40-type NLS is sufficient to direct the β-glucuronidase reporter to the nucleus of transiently transformed tobacco protoplasts, but is dispensible for nuclear localization of both proteins. The dominant and essential NLS in PS-IAA4 and PS-IAA6 overlap with a bipartite basic motif which is polymorphic and conserved in related proteins from other plant species, having the consensus sequence (KKNEK)KR-X(2471)-(RSXRK)/(RK/RK). Both basic elements of this motif in PS-IAA4, (KR-X41-RSYRK), function interdependently as a bipartite NLS. However, in PS-IAA6 (KKNEKKR-X36-RKK) the upstream element of the corresponding motif contains additional basic residues which allow its autonomous function as an SV40-type monopartite NLS. The spacer-length polymorphism, X(2470), in respective bipartite NLS peptides of several PS-IAA4-like proteins from Arabidopsis thaliana does not affect nuclear targeting function. The structural and functional variation of the bipartite basic motif in PS-IAA4-like proteins supports the proposed integrated consensus of NLS.

Publikation

Harms, K., Atzorn, R., Brash, A., Kühn, H., Wasternack, C., Willmitzer, L. & Peña-Cortés, H. Expression of a flax allene oxide synthase cDNA leads to an increase in the endogenous jasmonic acid level in transgenic potato plants but not to a corresponding activation of jasmonic acid-responding genes The Plant Cell 7, 1645-1654, (1995)

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Publikation

Wasternack, C., Atzorn, R., Leopold, J., Feussner, I., Rademacher, W. & Parthier, B. Synthesis of jasmonate-induced proteins in barley (Hordeum vulgare) is inhibited by the growth retardant tetcyclacis Physiol. Plantarum 94, 335-341, (1995)

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Publikation

Kogel, K.-H., Ortel, B., Jarosch, B., Atzorn, R., Schiffer, R. & Wasternack, C. Resistance in barley against the powdery mildew fungus (Erysiphe graminis f. sp. hordei) is not associated with enhanced levels of endogenous jasmonates Eur. J. Plant Pathol. 101, 319-332, (1995)

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Publikation

Abel, S., Nguyen, M.D. & Theologis, A. The PS-IAA4/5-like family of early auxin-inducible mRNAs in Arabidopsis thaliana Journal of Biological Chemistry 270, 19093-19099, (1995)

1-Aminocyclopropane-1-carboxylic acid (ACC) synthase is the key regulatory enzyme in the biosynthetic pathway of the plant hormone ethylene. The enzyme is encoded by a divergent multigene family in Arabidopsis thaliana, comprising at least five genes, ACS1-5 (Liang, X., Abel, S., Keller, J. A., Shen, N. F., and Theologis, A.(1992) Proc. Natl. Acad. Sci. U. S. A. 89, 11046-11050). In etiolated seedlings, ACS4 is specifically induced by indoleacetic acid (IAA). The response to IAA is rapid (within 25 min) and insensitive to protein synthesis inhibition, suggesting that the ACS4 gene expression is a primary response to IAA. The ACS4 mRNA accumulation displays a biphasic dose-response curve which is optimal at 10 μM of IAA. However, IAA concentrations as low as 100 nM are sufficient to enhance the basal level of ACS4 mRNA. The expression of ACS4 is defective in the Arabidopsis auxin-resistant mutant lines axr1-12, axr2-1, and aux1-7. ACS4 mRNA levels are severely reduced in axr1-12 and axr2-1 but are only 1.5-fold lower in aux1-7. IAA inducibility is abolished in axr2-1. The ACS4 gene was isolated and structurally characterized. The promoter contains four sequence motifs reminiscent of functionally defined auxin-responsive cis-elements in the early auxin-inducible genes PS-IAA4/5 from pea and GH3 from soybean. Conceptual translation of the coding region predicts a protein with a molecular mass of 53,795 Da and a theoretical isoelectric point of 8.2. The ACS4 polypeptide contains the 11 invariant amino acid residues conserved between aminotransferases and ACC synthases from various plant species. An ACS4 cDNA was generated by reverse transcriptase-polymerase chain reaction, and the authenticity was confirmed by expression of ACC synthase activity in Escherichia coli.

Publikation

Kramell, R., Atzorn, R., Schneider, G., Miersch, O., Brückner, C., Schmidt, J., Sembdner, G. & Parthier, B. Occurrence and identification of jasmonic acid and its amino acid conjugates induced by osmotic stress in barley leaf tissue J. Plant Growth Reg. 14, 29-36, (1995)

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Publikation

Lehmann, J., Atzorn, R., Brückner, C., Reinbothe, S., Leopold, J., Wasternack, C. & Parthier, B. Accumulation of jasmonate, abscisic acid, specific transcripts and proteins in osmotically stressed barley leaf segments Planta 197, 156-162, (1995)

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