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Publikationen - Molekulare Signalverarbeitung

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White, M. D., Klecker, M., Hopkinson, R. J., Weits, D. A., Mueller, C., Naumann, C., O’Neill, R., Wickens, J., Yang, J., Brooks-Bartlett, J. C., Garman, E. F., Grossmann, T. N., Dissmeyer, N. & Flashman, E. Plant cysteine oxidases are dioxygenases that directly enable arginyl transferase-catalysed arginylation of N-end rule targets Nature Commun 8, 14690, (2017) DOI: 10.1038/ncomms14690

Crop yield loss due to flooding is a threat to food security. Submergence-induced hypoxia in plants results in stabilization of group VII ETHYLENE RESPONSE FACTORs (ERF-VIIs), which aid survival under these adverse conditions. ERF-VII stability is controlled by the N-end rule pathway, which proposes that ERF-VII N-terminal cysteine oxidation in normoxia enables arginylation followed by proteasomal degradation. The PLANT CYSTEINE OXIDASEs (PCOs) have been identified as catalysts of this oxidation. ERF-VII stabilization in hypoxia presumably arises from reduced PCO activity. We directly demonstrate that PCO dioxygenase activity produces Cys-sulfinic acid at the N terminus of an ERF-VII peptide, which then undergoes efficient arginylation by an arginyl transferase (ATE1). This provides molecular evidence of N-terminal Cys-sulfinic acid formation and arginylation by N-end rule pathway components, and a substrate of ATE1 in plants. The PCOs and ATE1 may be viable intervention targets to stabilize N-end rule substrates, including ERF-VIIs, to enhance submergence tolerance in agriculture.
Bücher und Buchkapitel

Hellmuth, A. & Calderón Villalobos, L. I. A. Radioligand Binding Assays for Determining Dissociation Constants of Phytohormone Receptors. In: Plant Proteostasis   (Lois, L. M.; Matthiesen, R. ). Meth. Mol. Biol 1450, 23-34, (2016) ISBN: 978-1-4939-3757-8 DOI: 10.1007/978-1-4939-3759-2_3

In receptor–ligand interactions, dissociation constants provide a key parameter for characterizing binding. Here, we describe filter-based radioligand binding assays at equilibrium, either varying ligand concentrations up to receptor saturation or outcompeting ligand from its receptor with increasing concentrations of ligand analogue. Using the auxin coreceptor system, we illustrate how to use a saturation binding assay to determine the apparent dissociation constant (K D ′ ) for the formation of a ternary TIR1–auxin–AUX/IAA complex. Also, we show how to determine the inhibitory constant (K i) for auxin binding by the coreceptor complex via a competition binding assay. These assays can be applied broadly to characterize a one-site binding reaction of a hormone to its receptor.

Bücher und Buchkapitel

Liu, S., Kracher, B., Ziegler, J., Birkenbihl, R. P. & Somssich, I. E. Negative regulation of ABA signaling by WRKY33 is critical for Arabidopsis immunity towards Botrytis cinerea 2100. In: eLife 4, e07295, (2015) DOI: 10.7554/eLife.07295

The Arabidopsis mutant wrky33 is highly susceptible to Botrytis cinerea. We identified >1680 Botrytis-induced WRKY33 binding sites associated with 1576 Arabidopsis genes. Transcriptional profiling defined 318 functional direct target genes at 14 hr post inoculation. Comparative analyses revealed that WRKY33 possesses dual functionality acting either as a repressor or as an activator in a promoter-context dependent manner. We confirmed known WRKY33 targets involved in hormone signaling and phytoalexin biosynthesis, but also uncovered a novel negative role of abscisic acid (ABA) in resistance towards B. cinerea 2100. The ABA biosynthesis genes NCED3 and NCED5 were identified as direct targets required for WRKY33-mediated resistance. Loss-of-WRKY33 function resulted in elevated ABA levels and genetic studies confirmed that WRKY33 acts upstream of NCED3/NCED5 to negatively regulate ABA biosynthesis. This study provides the first detailed view of the genome-wide contribution of a specific plant transcription factor in modulating the transcriptional network associated with plant immunity.

Bücher und Buchkapitel

Wasternack, C. Jasmonates in plant growth and stress responses.. In: Phytohormones: a window to metabolism, signaling and biotechnological applications. (Tran, L.-S.; Pal, S.). Springer, 221-264, (2014) ISBN: 978-1-4939-0490-7 (hardcover) 978-1-4939-4814-7 (softcover) DOI: 10.1007/978-1-4939-0491-4_8

Abiotic and biotic stresses adversely affect plant growth and productivity. The phytohormones regulate key physiological events under normal and stressful conditions for plant development. Accumulative research efforts have discovered important roles of phytohormones and their interactions in regulation of plant adaptation to numerous stressors. Intensive molecular studies have elucidated various plant hormonal pathways; each of which consist of many signaling components that link a specific hormone perception to the regulation of downstream genes. Signal transduction pathways of auxin, abscisic acid, cytokinins, gibberellins and ethylene have been thoroughly investigated. More recently, emerging signaling pathways of brassinosteroids, jasmonates, salicylic acid and strigolactones offer an exciting gateway for understanding their multiple roles in plant physiological processes.

At the molecular level, phytohormonal crosstalks can be antagonistic or synergistic or additive in actions. Additionally, the signal transduction component(s) of one hormonal pathway may interplay with the signaling component(s) of other hormonal pathway(s). Together these and other research findings have revolutionized the concept of phytohormonal studies in plants. Importantly, genetic engineering now enables plant biologists to manipulate the signaling pathways of plant hormones for development of crop varieties with improved yield and stress tolerance.

This book, written by internationally recognized scholars from various countries, represents the state-of-the-art understanding of plant hormones’ biology, signal transduction and implications. Aimed at a wide range of readers, including researchers, students, teachers and many others who have interests in this flourishing research field, every section is concluded with biotechnological strategies to modulate hormone contents or signal transduction pathways and crosstalk that enable us to develop crops in a sustainable manner. Given the important physiological implications of plant hormones in stressful environments, our book is finalized with chapters on phytohormonal crosstalks under abiotic and biotic stresses. 
Bücher und Buchkapitel

Tissier, A., Ziegler, J. & Vogt T. Specialized plant metabolites: Diversity and biosynthesis . In: Ecological Biochemistry: environmental and Interspecies Interactions (Krauß, G. J.; Nies, D. H.). 14-37, (2014) ISBN: 978-3-527-31650-2 DOI: 10.1002/9783527686063.ch2

Plant secondary metabolites, also termed specialized plant metabolites, currently comprise more than 200 000 natural products that are all based on a few biosynthetic pathways and key primary metabolites. Some pathways like flavonoid and terpenoid biosynthesis are universally distributed in the plant kingdom, whereas others like alkaloid or cyanogenic glycoside biosynthesis are restricted to a limited set of taxa. Diversification is achieved by an array of mechanisms at the genetic and enzymatic level including gene duplications, substrate promiscuity of enzymes, cell-specific regulatory systems, together with modularity and combinatorial aspects. Specialized metabolites reflect adaptations to a specific environment. The observed diversity illustrates the heterogeneity and multitude of ecological habitats and niches that plants have colonized so far and constitutes a reservoir of potential new metabolites that may provide adaptive advantage in the face of environmental changes. The code that connects the observed chemical diversity to this ecological diversity is largely unknown. One way to apprehend this diversity is to realize its tremendous plasticity and evolutionary potential. This chapter presents an overview of the most widespread and popular secondary metabolites, which provide a definite advantage to adapt to or to colonize a particular environment, making the boundary between the “primary” and the “secondary” old fashioned and blurry.
Bücher und Buchkapitel

Wasternack, C. & Hause, B. Benno Parthier und die Jasmonatforschung in Halle. In: Benno Parthier und die Jasmonatforschung in Halle Nova Acta Leopoldina, NF Supplementum 28, 29-38, (2013) ISBN: ISBN 978-3-8047-3209-4

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Bücher und Buchkapitel

Carbonell, A., Flores, R. & Gago, S. Hammerhead Ribozymes Against Virus and Viroid RNAs. In: From Nucleic Acids Sequences to Molecular Medicine (Erdmann, V. A., Barciszewski, J.). Springer-Verlag Berlin Heidelberg 411-427, (2012)

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Bücher und Buchkapitel

Vaira, A.M., Gago-Zachert, S., Garcia, M.L., Guerri, J., Hammond, J., Milne, R.G., Moreno, P., Morikawa, T., Natsuaki, T., Navarro, J.A., Pallas, V., Torok, V., Verbeek, M. & Vetten, H.J. Family Ophioviridae. In: Virus Taxonomy. Ninth Report of the International Committee on Taxonomy of Viruses (King, A. M. Q., Adams, M. J., Carstens, E. B., Lefkowitz, E. J.). Elsevier, Academic Press 743-748, (2011) ISBN: 978-0-12-384684-6

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Bücher und Buchkapitel

Yamaguchi, I., Cohen, J.D., Culler, A.H., Quint, M., Slovin, J.P., Nakajima, M., Sakakibara, H., Kuroha, T., Hirai, N., Yokota, T., Ohta, H., Kabayashi, Y., Mori, H. & Sakagami, Y. Plant Hormones. In: Comprehensive Natural Products II (Lew Mander and Hung-Wen (Ben) Liu). Comprehensive Natural Products II, Elsevier, Oxford 9-125, (2010)

The definition of a plant hormone has not been clearly established, so the compounds classified as plant hormones often vary depending on which definition is considered. In this chapter, auxins, gibberellins (GAs), cytokinins, abscisic acid, brassinosteroids, jasmonic acid-related compounds, and ethylene are described as established plant hormones, while polyamines and phenolic compounds are not included. On the other hand, several peptides that have been proven to play a clear physiological role(s) in plant growth and development, similar to the established plant hormones, are referred. This chapter will focus primarily on the more recent discoveries of plant hormones and their impact on our current understanding of their biological role. In some cases, however, it is critical to place recent work in a proper historical context.

Bücher und Buchkapitel

Wasternack, C. Jasmonates in Stress, Growth, and Development. In: Plant Stress Biology (H. Hirt). WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim 91 - 118, (2009) ISBN: 978-3-527-32290-9

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