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Publikationen - Molekulare Signalverarbeitung

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Publikation

Floková, K., Feussner, K., Herrfurth, C., Miersch, O., Mik, V., Tarkowská, D., Strnad, M., Feussner, I., Wasternack, C. & Novák, O. A previously undescribed jasmonate compound in flowering Arabidopsis thaliana – The identification of cis-(+)-OPDA-Ile. Phytochemistry 122, 230-237, (2016) DOI: 10.1016/j.phytochem.2015.11.012

Jasmonates (JAs) are plant hormones that integrate external stress stimuli with physiological responses. (+)-7-iso-JA-L-Ile is the natural JA ligand of COI1, a component of a known JA receptor. The upstream JA biosynthetic precursor cis-(+)-12-oxo-phytodienoic acid (cis-(+)-OPDA) has been reported to act independently of COI1 as an essential signal in several stress-induced and developmental processes. Wound-induced increases in the endogenous levels of JA/JA-Ile are accompanied by two to tenfold increases in the concentration of OPDA, but its means of perception and metabolism are unknown. To screen for putative OPDA metabolites, vegetative tissues of flowering Arabidopsis thaliana were extracted with 25% aqueous methanol (v/v), purified by single-step reversed-phase polymer-based solid-phase extraction, and analyzed by high throughput mass spectrometry. This enabled the detection and quantitation of a low abundant OPDA analog of the biologically active (+)-7-iso-JA-L-Ile in plant tissue samples. Levels of the newly identified compound and the related phytohormones JA, JA-Ile and cis-(+)-OPDA were monitored in wounded leaves of flowering Arabidopsis lines (Col-0 and Ws) and compared to the levels observed in Arabidopsis mutants deficient in the biosynthesis of JA (dde2-2, opr3) and JA-Ile (jar1). The observed cis-(+)-OPDA-Ile levels varied widely, raising questions concerning its role in Arabidopsis stress responses.

Publikation

Churin, J., Hause, B., Feussner, I., Maucher, H.P., Feussner, K., Börner, T. & Wasternack, C. Cloning and expression of a new cDNA from monocotyledonous plants coding for a diadenosine 5,5'-P1,P4-tetraphosphate hydrolase from barley (Hordeum vulgare) FEBS Letters 431, 481-485, (1998)

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Publikation

Feussner, K., Feussner, I., Leopold, I. & Wasternack, C. Isolation of a cDNA coding for an ubiquitin-conjugating enzyme UBCI of tomato - the first stress-induced UBC of higher plants FEBS Letters 409, 211-215, (1997)

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Publikation

Feussner, K., Guranowski, A., Kostka, S. & Wasternack, C. Diadenosine 5'5'''-P1,P4-tetraphosphate (Ap4A) hydrolase from tomato (Lycopersicon esculentum cv. Lukullus) - Purification, Biochemical properties and behaviour during stress Z. Naturforsch. 51c, 477-486, (1996)

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