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Publikationen - Molekulare Signalverarbeitung

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Bücher und Buchkapitel

Carbonell, A., Flores, R. & Gago, S. Hammerhead Ribozymes Against Virus and Viroid RNAs. In: From Nucleic Acids Sequences to Molecular Medicine (Erdmann, V. A., Barciszewski, J.). Springer-Verlag Berlin Heidelberg 411-427, (2012)

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Publikation

Carbonell, A., Flores, R. & Gago, S. Trans-cleaving hammerhead ribozymes with tertiary stabilizing motifs: in vitro and in vivo activity against a structured viroid RNA. Nucleic Acids Res. 39, 2432-44, (2011) DOI: 10.1093/nar/gkq1051

Trans-cleaving hammerheads with discontinuous or extended stem I and with tertiary stabilizing motifs (TSMs) have been tested previously against short RNA substrates in vitro at low Mg2+ concentration. However, the potential of these ribozymes for targeting longer and structured RNAs in vitro and in vivo has not been examined. Here, we report the in vitro cleavage of short RNAs and of a 464-nt highly structured RNA from potato spindle tuber viroid (PSTVd) by hammerheads with discontinuous and extended formats at submillimolar Mg2+. Under these conditions, hammerheads derived from eggplant latent viroid and peach latent mosaic viroid (PLMVd) with discontinuous and extended formats, respectively, where the most active. Furthermore, a PLMVd-derived hammerhead with natural TSMs showed activity in vivo against the same long substrate and interfered with systemic PSTVd infection, thus reinforcing the idea that this class of ribozymes has potential to control pathogenic RNA replicons.

Publikation

Carbonell, A., Flores, R. & Gago, S. In vivo and in vitro activity against a structured viroid RNA of trans-cleaving hammerhead ribozymes with tertiary stabilizing motifs Nucleic Acids Research 39, 2432-2444, (2011)

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Publikation

Flores, R., Gas, M.E., Molina-Serrano, D., Nohales, M.A., Carbonell, A., Gago, S., de la Peña, M. & Daròs, J.A. Viroid replication: rolling-circles, enzymes and ribozymes Viruses 1, 317-334, (2009) DOI: 10.3390/v1020317

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Bücher und Buchkapitel

Flores, R., Carbonell, A., Gago, S., Martínez de Alba, A.E., Delgado, S., Rodio, M.E. & di Serio, F. Viroid-host interactions: A molecular dialogue between two uneven partners. In: Biology of Plant-Microbe Interactions (Lorito, M., Woo, S. L., Scala, F.). 6 (chap. 58), 1-9, (2008)

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Publikation

Carbonell, A., Martínez de Alba, A.E., Flores, R. & Gago, S. Double-stranded RNA interferes in a sequence-specific manner with the infection of representative members of the two viroid families Virology 371, 44-53, (2008)

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Bücher und Buchkapitel

Flores, R., Carbonell, A., de la Peña, M. & Gago, S. RNAs autocatalíticos: ribozimas de cabeza de martillo. In: Herramientas Biotecnológicas en Fitopatología (Fenoll, C., Marcos, J., Pallás, V., Rodriguez Palenzuela, P.). Spanish Society of Phytopathology 407-420, (2007) ISBN: 84-6476-319-6

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Publikation

Carbonell, A., de la Peña, M., Flores, R. & Gago, S. Effects of the trinucleotide preceding the self-cleavage site on eggplant latent viroid hammerheads: difference in co- and post-transcriptional self-cleavage may explain the lack trinucleotide AUC in most natural hammerheads Nucleic Acids Research 34, 5613-5622, (2006)

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Publikation

Flores, R., Delgado, S., Gas, M.E., Carbonell, A., Molina, D., Gago, S. & de la Peña, M. Viroids: the minimal non-coding RNA's with autonomous replication FEBS Letters 567, 42-48, (2004)

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