Publications - Cell and Metabolic Biology
Search narrowed by
Advanced Search
- Type of publication
- Publication (1)
- Year
- 1998 (1)
- Journal / Volume / Preprint Server Sorted by frequency and by alphabetical order
- Biochemistry (1)
- Author Sorted by frequency and by alphabetical order
- Hause, B. (73)
- Strack, D. (46)
- Tissier, A. (29)
- Vogt, T. (21)
- Marillonnet, S. (20)
- Wasternack, C. (18)
- Milkowski, C. (16)
- Schmidt, J. (15)
- Stenzel, I. (12)
- Schliemann, W. (11)
- Hause, G. (10)
- Miersch, O. (9)
- Walter, M. H. (9)
- Fester, T. (8)
- Brandt, W. (7)
- Athmer, B. (6)
- Baumert, A. (6)
- Bergau, N. (6)
- Feussner, I. (6)
- Franken, P. (6)
- Frolov, A. (6)
- Wray, V. (6)
- Balcke, G. U. (5)
- Fellenberg, C. (5)
- Krajinski, F. (5)
- Kretschmer, C. (5)
- Maucher, H. (5)
- Ordon, J. (5)
- Schaarschmidt, S. (5)
- Scheel, D. (5)
- Schreiber, T. (5)
- Stuttmann, J. (5)
- Wessjohann, L. (5)
- Ziegler, J. (5)
- Bennewitz, S. (4)
- Böttcher, C. (4)
- Fraser, P. D. (4)
- Hoehenwarter, W. (4)
- Lee, J. (4)
- Peumans, W. J. (4)
- Porzel, A. (4)
- Roitsch, T. (4)
- Van Damme, E. J. M. (4)
- Bouwmeester, H. (3)
- Bucher, M. (3)
- Büttner, D. (3)
- Druege, U. (3)
- Gantner, J. (3)
- Gruber, C. (3)
- Handrick, V. (3)
- Hoffmann, R. (3)
- Isayenkov, S. (3)
- Kramell, R. (3)
- Küster, H. (3)
- Lischewski, S. (3)
- Lohse, S. (3)
- Löfke, C. (3)
- Schmidt, D. (3)
- Schneider, B. (3)
- Schröder, G. (3)
- Schröder, J. (3)
- Warzecha, H. (3)
- Adam, G. (2)
- Ahkami, A. H. (2)
- Ammer, C. (2)
- Balcke, G. (2)
- Barre, A. (2)
- Bassi, R. (2)
- Becker, H. C. (2)
- Berger, S. (2)
- Bilova, T. (2)
- Born, R. (2)
- Brand, A. (2)
- Bressan, M. (2)
- Böhm, H. (2)
- Bürstenbinder, K. (2)
- Dagdas, Y. (2)
- Darwish, E. (2)
- Dhakarey, R. (2)
- Dunker, F. (2)
- Eckermann, S. (2)
- Ehrlich, H. (2)
- Eschen-Lippold, L. (2)
- Fischer, C. (2)
- Floss, D. S. (2)
- Gruetzner, R. (2)
- Göbel, C. (2)
- Hajirezaei, M. R. (2)
- Halfmann, K. (2)
- Hanke, T. (2)
- Hans, J. (2)
- Hausner, J. (2)
- Heilmann, I. (2)
- Henle, T. (2)
- Hohlfeld, H. (2)
- Hu, J. (2)
- Humbeck, K. (2)
- Hückelhoven, R. (2)
- Hüsken, A. (2)
- Ilse, T. (2)
Displaying results 1 to 1 of 1.
Schröder, J.; Raiber, S.; Berger, T.; Schmidt, A.; Schmidt, J.; Soares-Sello, A. M.; Bardshiri, E.; Strack, D.; Simpson, T. J.; Veit, M.; Schröder, G.; Plant Polyketide Synthases: A Chalcone Synthase-Type Enzyme Which Performs a Condensation Reaction with Methylmalonyl-CoA in the Biosynthesis of C-Methylated Chalcones Biochemistry 37, 8417-8425, (1998) DOI: 10.1021/bi980204g
Heterologous screening of a cDNA library from Pinus strobus seedlings identified clones for two chalcone synthase (CHS) related proteins (PStrCHS1 and PStrCHS2, 87.6% identity). Heterologous expression in Escherichia coli showed that PStrCHS1 performed the typical CHS reaction, that it used starter CoA-esters from the phenylpropanoid pathway, and that it performed three condensation reactions with malonyl-CoA, followed by the ring closure to the chalcone. PstrCHS2 was completely inactive with these starters and also with linear CoA-esters. Activity was detected only with a diketide derivative (N-acetylcysteamine thioester of 3-oxo-5-phenylpent-4-enoic acid) that corresponded to the CHS reaction intermediate postulated after the first condensation reaction. PstrCHS2 performed only one condensation, with 6-styryl-4-hydroxy-2-pyrone derivatives as release products. The enzyme preferred methylmalonyl-CoA against malonyl-CoA, if only methylmalonyl-CoA was available. These properties and a comparison with the CHS from Pinussylvestris suggested for PstrCHS2 a special function in the biosynthesis of secondary products. In contrast to P. sylvestris, P. strobus contains C-methylated chalcone derivatives, and the methyl group is at the position predicted from a chain extension with methylmalonyl-CoA in the second condensation of the biosynthetic reaction sequence. We propose that PstrCHS2 specifically contributes the condensing reaction with methylmalonyl-CoA to yield a methylated triketide intermediate. We discuss a model that the biosynthesis of C-methylated chalcones represents the simplest example of a modular polyketide synthase.