Untersuchungen zur Funktion und Spezifität von Glycosyltransferasen

ANDREAS BECHTHOLD
Albert-Ludwigs-Universität Freiburg
Institut für Pharmazeutische Biologie
Stefan-Meier-Straße 19
D-79104 Freiburg
andreas.bechthold@pharmazie.uni-freiburg.de
http://www.biologie.uni-freiburg.de/

GEORG E. SCHULZ
Albert-Ludwigs-Universität Freiburg
Institut für Organische Chemie und Biochemie
Albertstraße 21
D-79104 Freiburg
schulz@bio.chemie.uni-freiburg.de
http://bio5.chemie.uni-freiburg.de/main.html

References
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Zhu L, Ostash, B., Rix, U., Nur-E-Alam, M., Mayers, A., Luzhetskyy, A., Mendez, C., Salas, J.A., Bechthold, A., Fedorenko, V., Rohr, J. 2005. Identification of the function of gene lndM2 encoding a bifunctional oxygenase-reductase involved in the biosynthesis of the antitumor antibiotic landomycin E by Streptomyces globisporus 1912 supports the originally assigned structure for landomycinone. J Org Chem. 21,70(2): 631-638

Luzhetskyy, A., Taguchi, T., Fedoryshyn, M., Dürr, C., Wohlert, S., Novikov, V., Bechthold, A. 2005. LanGT2 catalyzes the first glycosylation step during landomycin A biosynthesis. ChemBioChem. 6(8):1406-1410

Luzhetskyy, A., Fedoryshyn, M., Dürr, C., Taguchi, T., Novikov, V., Bechthold, A. 2005. Iteratively acting glycosyltransferases involved in the hexasaccharide biosynthesis of landomycin A. Chem Biol. 12(7): 725-729.

Lutzhetskyy, A., Bechthold, A. 2005. It works: Combinatorial biosynthesis for generating novel glycosylated compounds. Mol Microbiol. 58: 3-5.

Hofmann, C., Boll, R., Heitmann, B., Hauser, G., Dürr, C., Frerich, A., Weitnauer, G., Glaser, S.J., Bechthold, A. 2005. Identification of genes encoding enzymes responsible for the biosynthesis of L-lyxose and the attachment of methyleurkanate during avilamycin biosynthesis. Chem Biol. 12(10): 1137-43.

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Berner, M., Krug, D., Bihlmaier, C., Vente, A., Müller, R., Bechthold, A. 2006. Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix espanaensis. J. Bacteriol., 188(7): 2666-73.

Boll, R., Hofmann, C., Heitmann, B., Hauser, G., Glaser, S., Koslowski, T., Friedrich, T., Bechthold, A. 2006. The active Conformation of Avilamycin A is Confered by AviX12, a Radical SAM Enzyme. JBC, 281(21): 14756-63.

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Zhu, L., Luzhetskyy, A., Mattingly, C., Adams, V., Bechthold, A., Rohr, J. 2007. Generation of new landomycins with altered saccharide pattern through overexpression of the glycosyltransferase gene lanGT3 in the biosynthetic gene cluster of landomycin A in Streptomyces cyanogenus S136. ChemBioChem, 2;8(1): 83-88.

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Luzhetskyy, A., Meyer, A., Hoffmann, J., Pelzer, S., Wohlert, S.-E., Vente, A., Bechthold, A. 2006. Cloning and heterologous expression of the aranciamycin biosynthetic gene cluster revealed a new flexible glycosyltransferase. ChemBioChem 8 (6): 599-602.

Fedoryshyn, M., Nur-e-Alam, M, Zhu, L., Luzhetskyy, A., Rohr, J., Bechthold, A. 2007. Surprising production of a new urdamycin derivative by S. fradiae D urdQ/R. J. of Biotechnology 130: 32-38.

Kopp, M., Rupprath, C., Irschik, H., Bechthold, A., Elling, L., Müller, R. 2007. SorF: A glycosyltransferase with promiscuous donor substrate specificity in vitro. ChemBioChem 7 (8): 813-819.

Hornung, A., Bertazzo, M., Dziarnowski, A., Schneider, K., Welzel, K., Wohlert, S.-E., Holzenkämpfer, M., Nicholson, G.J., Bechthold, A., Süssmuth, R., Vente, A., Pelzer, S. 2007. A Genomic Screening Approach to the Structure-Guided Identification of Drug Candidates from Natural Sources. ChemBioChem 7 (8): 757-766.

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